권호기사보기
기사명 | 저자명 | 페이지 | 원문 | 기사목차 |
---|
대표형(전거형, Authority) | 생물정보 | 이형(異形, Variant) | 소속 | 직위 | 직업 | 활동분야 | 주기 | 서지 | |
---|---|---|---|---|---|---|---|---|---|
연구/단체명을 입력해주세요. |
|
|
|
|
|
* 주제를 선택하시면 검색 상세로 이동합니다.
Title Page
Contents
ABSTRACT 10
INTRODUCTION 12
MATERIALS & METHODS 15
1. Cell culture 15
2. Three-Dimensional culture 15
3. Immunohistochemistry (IHC) 16
4. Transfection of Small interfering RNA (siRNA) 16
5. Transfection of miRNA precursors & inhibitors 17
6. RNA isolation 17
7. MiRNA expression analysis 17
8. Western blotting analysis 18
9. 5-Aza-2'-deoxycytidine treatment 19
10. Purification of genomicDNA and bisulfite treatmentn 19
11. Methylation analysis (Pyrosequencing & MS-HRM assay) 19
12. Lentiviral microRNA systems 21
13. Immunofluorescence 21
14. Statistical analysis 22
RESULTS 23
1. Epithelial-to-mesenchymal transition (EMT) is associated with cyst development in ADPKD 23
2. miR-192 and miR-194 are downregulated in vivo in kidney tissues of both human ADPKD patients and Pkd1 conditional knockout (cKO) mice 29
3. miR-192/215 and miR-194 influence cyst growth 33
4. Epithelial-to-mesenchymal transition (EMT)-associated genes are differentially expressed by miR-192/215 and miR-194 36
5. Downregulation of miR-192 and miR-194 is related to DNA hypermethylation 39
6. Overexpression of miR-192 and miR-194 influences cyst progression in vivo 47
DISCUSSION 55
REFERENCES 58
ABSTRACT IN KOREAN 60
Figure 1. Cyst development of Pkd1 conditional knockout (cKO) mice at all over the age. 25
Figure 2. The differential protein expression levels of epithelial-to-mesenchymal transition (EMT)-associated genes in P13 of Pkd1... 27
Figure 3. Inhibition of cyst growth by knockdown of epithelial-to-mesenchymal transition (EMT)-associated genes. 28
Figure 4. The expression levels of miR-192 and miR-194 in human kidney tissues. 30
Figure 5. The expression levels of miR-192 and miR-194 in Pkd1 conditional knockout (cKO) mice. 31
Figure 6. The expression levels of miR-192 and miR-194 in collecting duct cells isolated from the kidneys of Pkd1 conditional knockout (cKO) mouse. 32
Figure 7. Accelerated cyst growth by inhibition of miR-192/215 and miR-194. 34
Figure 8. Repression of cyst growth by overexpression of miR-192/215 and miR-194. 35
Figure 9. The differential expression of epithelial-to-mesenchymal transition (EMT)-associated genes by miR-192/215 and miR-194 inhibition. 37
Figure 10. The differential protein expression of epithelial-to-mesenchymal transition (EMT)-associated genes by miR-192/215 and miR-194... 38
Figure 11. Bisulfite pyrosequencing analysis of methylation level of hypermethylated MIR-192-194-2 in demethylating agent, 5-aza-dC,... 41
Figure 12. The normalized melting curves of the methylation-sensitive high resolution melting (MS-HRM) analysis of hypermethylated MIR-192-194-... 43
Figure 13. Mature and primary miR-192 and miR-194 expression levels upon 5-aza-dC treatment in WT9-12 cells. 44
Figure 14. The differential protein expression of epithelial-to-mesenchymal transition (EMT)-associated genes in 5-aza-dC treated WT9-12 cells. 45
Figure 15. Proposed schematic diagram on relation among miR-192/215 and miR-194, methylation, epithelial-to-mesenchymal transition (EMT), and... 46
Figure 16. The expression levels of miR-192 and miR-194 in collecting duct cells isolated from the kidneys of Pkd1 conditional knockout (cKO) mice. 49
Figure 17. The expression levels of miR-192 and miR-194 in lentivirus infected mIMCD cells. 50
Figure 18. Injection of lentiviral-overexpressed miR-192 and miR-194 in Pkd1 conditional knockout (cKO) mouse model. 51
Figure 19. The observation of cyst progression in Pkd1 conditional knockout (cKO) mice. 53
*표시는 필수 입력사항입니다.
전화번호 |
---|
기사명 | 저자명 | 페이지 | 원문 | 기사목차 |
---|
번호 | 발행일자 | 권호명 | 제본정보 | 자료실 | 원문 | 신청 페이지 |
---|
도서위치안내: / 서가번호:
우편복사 목록담기를 완료하였습니다.
*표시는 필수 입력사항입니다.
저장 되었습니다.