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Title Page
Contents
1. Introduction 8
2. Materials and Methods 11
2.1. Patient samples 11
2.2. RNA extraction, complementary DNA synthesis and relative quantitative reverse transcription polymerase chain reaction 11
2.3. JAK2 and CALR mutation analysis 12
2.4. Statistical analysis: 12
3. Results 15
3.1. Comparisons of the expression of miR-29a, miR-29b and miR-29c according to MPN subtypes and control 15
3.2. Comparisons the expression of miR-29a, miR-29b and miR-29c according to JAK2 V617F and CALR mutational status 15
3.3. Association of miR-29 expression with hematologic parameters or bone marrow fibrosis grade 16
4. Discussion 31
5. Summary 35
References 36
Abstract 40
초록 42
저자 약력 44
논문 및 저서 44
Figure 1. The comparisons of expression of miR-29a among PV, ET, PMF and normal control groups 17
Figure 2. The comparisons of expression of miR-29b among PV, ET, PMF and normal control groups 18
Figure 3. The comparisons of expression of miR-29c among PV, ET, PMF and normal control groups 19
Figure 4. The relative expression of miR-29 family in PV, ET, PMF compared to normal control 20
Figure 5. The comparison of expression of miR-29a between JAK2V617F positive and negative MPN patients 21
Figure 6. The comparison of expression of miR-29b between JAK2V617F positive and negative MPN patients 22
Figure 7. The comparison of expression of miR-29c between JAK2V617F positive and negative MPN patient 23
Figure 8. The comparison of expression of miR-29a between CALR positive and negative MPN patients 24
Figure 9. The comparison of expression of miR-29b between CALR positive and negative MPN patients 25
Figure 10. The comparison of expression of miR-29c between CALR positive and negative MPN patients 26
Figure 11. The correlations between WBC count and expression of (A) miR-29a, (B) miR-29b and (C) miR-29c in MPN patients 27
Figure 12. The correlations between hemoglobin leveland expression of (A) miR-29a, (B) miR-29b and (C) miR-29c in MPN patients 28
Figure 13. The correlations between platelet count and expression of (A) miR-29a, (B) miR-29b and (C) miR-29c in MPN patients 29
Figure 14. The relative expression of miR-29a,miR-29b and miR-29c compared to normal control in three groups according to bone... 30
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