본문바로가기

자료 카테고리

전체 1
도서자료 0
학위논문 1
연속간행물·학술기사 0
멀티미디어 0
동영상 0
국회자료 0
특화자료 0

도서 앰블럼

전체 (0)
일반도서 (0)
E-BOOK (0)
고서 (0)
세미나자료 (0)
웹자료 (0)
전체 (1)
학위논문 (1)
전체 (0)
국내기사 (0)
국외기사 (0)
학술지·잡지 (0)
신문 (0)
전자저널 (0)
전체 (0)
오디오자료 (0)
전자매체 (0)
마이크로폼자료 (0)
지도/기타자료 (0)
전체 (0)
동영상자료 (0)
전체 (0)
외국법률번역DB (0)
국회회의록 (0)
국회의안정보 (0)
전체 (0)
표·그림DB (0)
지식공유 (0)
전체 1
국내공공정책정보
국외공공정책정보
국회자료

도서 앰블럼

전체 ()
정부기관 ()
지방자치단체 ()
공공기관 ()
싱크탱크 ()
국제기구 ()
전체 ()
정부기관 ()
의회기관 ()
싱크탱크 ()
국제기구 ()
전체 ()
국회의원정책자료 ()
입법기관자료 ()

검색결과

검색결과 (전체 0건)

검색결과제한

열기
논문명/저자명
Study on the mechanism of Bfa1p to regulate mitotic exit network in budding yeast = 출아형 효모의 Bfa1p 단백질에 의한 Mitotic exit network 조절 기작 연구 / Junwon Kim 인기도
발행사항
서울 : 연세대학교 대학원, 2006.2
청구기호
TD 572.8 K49s
형태사항
xiv, 185 p. ; 26 cm
자료실
전자자료
제어번호
KDMT1200643207
주기사항
학위논문(박사) -- 연세대학교 대학원, Biochemistry, 2006.2
원문
미리보기

목차보기더보기

title page

Contents

Abbreviations 14

I. Abstract 16

II. Introduction 18

Subject 1: The C-terminus of Bfa1p in budding yeast is essential to induce mitotic arrest in response to diverse checkpoint-activating signals 22

Abstract 23

1. Introduction 25

2. Materials and methods 28

2.1. Yeast strains, cultures, cell cycle synchronization 28

2.2. Construction of BFA1 deletion mutants 28

2.3. Microscopic techniques and FACS analysis 29

2.4. Two-hybrid analyses 30

2.5. Protein techniques 30

2.6. Random mutagenesis of plasmid DNA 31

3. Results 32

3.1. Bfa1-D8391-574, the 184 amino acids in the C-terminus, is sufficient to arrest mitosis exit in response to spindle damage(이미지참조) 32

3.2. Bfa1-D8391-574 is sufficient to induce mitotic arrest by spindle misorientation(이미지참조) 34

3.3. Bfa1-D8391-574 is sufficient to arrest mitosis in response to DNA damage(이미지참조) 35

3.4. Only D8 can aggravate the defect of Ddc5p mutations that is suppressed when Bfa1p is deleted 36

3.5. D8391-574 and D161-376 are two independent domains that induce mitotic arrest when overexpressed 37

3.6. Only Bfa1-D8391-574 is necessary for Bfa1p localization to the SPB(이미지참조) 40

3.7. The mutant of Bfa1-D8, D8E438K, is deprived of the checkpoint function of D8(이미지참조) 41

4. Discussion 43

국문요약 73

Subject 2: The GAP activity of Bfa1p and its phosphorylation by Cdc5p are not essential to regulate the mitotic exit network in budding yeast 75

Abstract 76

1. Introduction 78

2. Materials and methods 81

2.1. Yeast strains, cultures, and cell cycle synchronization 81

2.2. Random mutagenesis of plasmid DNA 81

2.3. GTPase activity assays 82

2.4. Microscopy and FACS analysis 82

2.5. Yeast two-hybrid analyses 83

2.6. Protein analyses 83

3. Results 84

3.1. Bfa1p-D8M413I does not cause mitotic arrest when overexpressed(이미지참조) 84

3.2. Bfa1pM413I interacts with Tem1p and Bub2p on the SPB(이미지참조) 85

3.3. The Bfa1pM413I-Bub2p complex lacks GAP activity(이미지참조) 86

3.4. The GAP activity of Bfa1p-Bub2p complex is not essential to control the MEN 90

3.5. Cdc5p does not phosphorylate Bfa1pM413I(이미지참조) 91

3.6. Cdc5p-mediated phosphorylation of Bfa1p is essential not to promote mitotic exit but to direct Bfa1p to the SPB toward bud 93

3.7. BFA1M413I cells fail to induce mitotic arrest in response to spindle damage(이미지참조) 95

3.8. BFA1M413I cells induce mitotic arrest in response to spindle mis-orientation and DNA damage(이미지참조) 96

3.9. Bfa1pMB mutant contains GAP activity with Bub2p but is not be phosphorylated in vivo(이미지참조) 97

4. Discussion 100

4.1. Triggering mitotic exit in budding yeast 100

4.2. Cdc5p plays a role in asymmetrically localizing Bfa1p on SPBdaughter at anaphase onset(이미지참조) 101

4.3. The GAP activity of Bfa1p-Bub2p complex is required to arrest the cell cycle in response to spindle damage 104

5. Supplementary materials 135

국문요약 149

Subject 3: The GAP activity of Bfa1p-Bub2p is responsible for mitotic arrest in response to spindle damage and Bfa1p overexpression, but not to spindle misorientation and DNA damage 151

Abstract 152

1. Introduction 154

2. Materials and methods 157

2.1. Yeast strains and random mutagenesis of plasmid DNA 157

2.2. Random mutagenesis of plasmid DNA 157

2.3. GTPase activity assays 158

2.4. Protein analyses 158

2.5. Yeast two-hybrid analyses 158

2.6. Microscopy and FACS analysis 159

3. Results 160

3.1. Bfa1p-D8E438K does not cause mitotic arrest when overexpressed(이미지참조) 160

3.2. Bfa1pE438K interacts with Tem1p and Bub2p on the SPB(이미지참조) 160

3.3. Bfa1pE438K is phosphorylated by Cdc5p(이미지참조) 161

3.4. The GAP activity of Bfa1pE438K-Bub2p is slightly decreased(이미지참조) 162

3.5. Bfa1pE438K-Bub2p complex is capable of regulating mitotic exit in similar kinetics to Bfa1p-Bub2p(이미지참조) 163

3.6. BFA1E438K cells induce mitotic arrest in response to diverse checkpoint-activating signals(이미지참조) 165

3.7. BFA1E438K suppresses the growth of cdc5-1△bfa1 mutant, but not of cdc5-2△bfa1 166

4. Discussion 168

국문요약 189

III. Reference 191

IV. 국문요약 198

Table 1. Yeast strains used in this study 48

Table 2. Oligonucleotides used to construct the Bfa1 deletion mutants 49

Table 3. Construction of the BFA1 deletion mutants 50

Table 1. Yeast strains used in this study 106

Supplementary tabel 1. Yeast strains used in the supplementary study 148

Table 1. Yeast strains used in this study 171

Figure 1. Summary of the functional domains of Bfa1p 51

Figure 2. The C-terminal 184 amino acids of Bfa1p, Bfa1-D8, bear the ability of Bfa1p to… 53

Figure 3. Bfa1-D8 bears the ability of Bfa1p to generate M arrest in response to spindle misorientation 57

Figure 4. Bfa1p-D8 bears the ability of Bfa1p to induce M arrest in response to DNA damage 60

Figure 5. Bfa1-D8 restores the mitotic exit defects of Cdc5p mutations that are suppressed in cdc3 bfa1△ 62

Figure 6. Bfa1 can be bipartite into the N-terminal 376 amino acids (Bfa1-D16) and the… 64

Figure 7. Bfa1-D8 bears the SPB localization activity of Bfa1p 69

Figure 8. The mutant of Bfa1-D8391-574, D8E438K, is deprived of the checkpoint function of D8 for MEN regulation(이미지참조) 71

Figure 1. Bfa1p-D8M413I does not induce mitotic arrest when overexpressed(이미지참조) 108

Figure 2. Bfa1pM413I interacts with Tem1p and Bub2p on the SPB(이미지참조) 111

Figure 3. The Bfa1 pM413I-Bub2p complex lacks GAP activity(이미지참조) 114

Figure 4. The GAP activity of the Bfa1p-Bub2p complex is not essential to control the MEN 117

Figure 5. Cdc5p does not phosphorylate Bfa1pM413I(이미지참조) 120

Figure 6. Cdc5p-mediated phosphorylation of Bfa1p is essential not to promote mitotic exit but to direct Bfa1p to the SPB toward bud(이미지참조) 122

Figure 7. Bfa1pM413I cannot inhibit mitotic exit in response to spindle damage(이미지참조) 124

Figure 8. Bfa1pM413I induces mitotic arrest in response to spindle mis-orientation and DNA damage(이미지참조) 127

Figure 9. The characterization of Bfa1pMB mutant(이미지참조) 130

Supplementary figure 1. The phenotype of full-length bfa1pM413I or Bfa1pMB overexpressing cells(이미지참조) 135

Supplementary figure 2. Interactions between Bfa1p-D8M413I and Tem1p or Bub2p were…(이미지참조) 136

Supplementary figure 3. The localization of Tem1p-RFP in BFA1 or △bfa1 cells 137

Supplementary figure 4. The GTPase activity of Tem1p was measured in the presence… 138

Supplementary figure 5. The quantitative analysis of the interactions between Bfa1pMB…(이미지참조) 141

Supplementary figure 6. Bfa1pMB inhibits mitotic exit in response to spindle damage(이미지참조) 142

Supplementary figure 7. Bfa1pMB induces mitotic arrest in response to spindle mis-orientation(이미지참조) 143

Supplementary figure 8. Bfa1pMB induces mitotic arrest in response to DNA damage(이미지참조) 144

Supplementary figure 9. Bfa1p-11A-GFP is localized on only SPBdaughter in anaphase(이미지참조) 145

Supplementary figure 10. Bfa1pMB cannot be efficiently phosphorylated by Cdc5p(이미지참조) 146

Supplementary figure 11. Ability of BFA1M413I and BFA1MB to alleviate the mitotic exit defect of cdc5-1 and cdc5-2(이미지참조) 147

Figure 1. Bfa1p-D8E438K does not cause mitotic arrest when overexpressed(이미지참조) 172

Figure 2. Bfa1pE438K interacts with Tem1p and Bub2p on the SPB(이미지참조) 175

Figure 3. Cdc5p phosphorylates Bfa1pE438K(이미지참조) 177

Figure 4. The GAP activity of Bfa1pE438K-Bub2p complex is slightly decreased(이미지참조) 179

Figure 5. Bfa1pE438K-Bub2p complex is capable of regulating mitotic exit in similar…(이미지참조) 181

Figure 6. Bfa1pE438K can inhibit mitotic exit in response to diverse checkpoint-activating signals(이미지참조) 183

Figure 7. BFA1E438K suppresses the growth of cdc5-1△bfa1 mutant, but not of cdc5-2△bfa1(이미지참조) 187

권호기사보기

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 기사목차
연속간행물 팝업 열기 연속간행물 팝업 열기