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ABSTRACT 6
Ⅰ. 緖論 9
Ⅱ. 實驗材料 및 方法 10
1. 산삼 약침의 조제 10
2. 실험동물 12
3. 종양세포의 배양 및 이식 12
4. 실험군의 분류 12
5. 체중, 종양 체적 및 종양 무게 측정 14
6. 말초혈액검사 14
7. 병리조직학적 검사 14
8. 고환의 정세관 직경의 측정 15
9. BrdU 면역조직화학 염색 15
10. 고환의 정세관당 정조세포수의 측정 15
11. BrdU 양성 반응 세포의 종류 및 BrdU 양성 정조세포비율의 측정 16
12. 고환의 TUNEL 염색 및 TUNEL 양성 정조세포수의 측정 16
13. 통계처리 16
Ⅲ. 實驗成績 18
1. 체중의 변화 18
2. 종양의 육안소견 20
3. 종양의 체적 및 무게 변화 22
4. 말초혈액 RBC Index 의 변화 23
5. 말초혈액 WBC 및 Platelets 의 변화 24
6. 병리조직학적 소견 25
(1) 종양조직 25
(2) 고환조직 28
7. 고환의 정세관 직경과 정세관당 정조세포 수의 변화 31
8. 고환조직의 BrdU 양성 반응 세포의 종류 32
9. 고환조직의 BrdU 정조세포수의 변화 35
10. 고환조직의 TUNEL 양성 반응 세포의 종류 36
11. 고환조직의 TUNEL 양성 정조세포 수의 변화 37
Ⅳ. 考察 38
Ⅴ. 結論 42
參考文獻 44
Table 1. Change of the body weight in C57BL/6 mice were injected with Cultivated Wild Ginseng Herbal Acupuncture after implantation of B16/F10 cells 18
Table 2. Change of the tumor volume and weight in C57BL/6 mice were injected with Cultivated Wild Ginseng Herbal Acupuncture after implantation of B16/F10 cells 22
Table 3. Change of the count of RBC, hemoglobin, hematocrit and reticulocyte in C57BL/6 mice were injected with Cultivated Wild Ginseng Herbal Acupuncture after implantation of B16/F10 cells. 23
Table 4. Change of the count of WBC, neutrophil, lymphocyte and platelet in C57BL/6 mice were injected with Cultivated Wild Ginseng Herbal Acupuncture after implantation of B16/F10 cells 24
Table 5. Change of the diameter of seminiferous tubules and count of spermatogonia in C57BL/6 mice were injected with Cultivated Wild Ginseng Herbal Acupuncture after implantation of B16/F10 cells. 31
Table 6. Change of the count of spermatogonia were labelled with BrdU in C57BL/6 mice were injected with Cultivated Wild Ginseng Herbal Acupuncture after implantation of B16/F10 cells 35
Table 7. Change of the count of spermatogonia were labelled with TUNEL in C57BL/6 mice were injected with Cultivated Wild Ginseng Herbal Acupuncture after implantation of B16/F10 cells 37
Fig. 1 The shapes of cultivated wild ginseng. 10
Fig. 2 Manufacturing process of distilled cultivated wild ginseng Herbal Acupuncture 11
Fig. 3 10 days after implantation of B16/F10 human melanoma cells Round shaped tumor mass was developed in subcutis of C57BL/6 mouse. 13
Fig. 4 19 days(control group at 9 days of experiment) after implantation of B16/F10 cells Note the large tumor mass and central scar tissue. 21
Fig. 5 19 days(Doxorubicin group at 9 days of experiment) after implantation of B16/F10 cells Ulceration and fistulation of the tumor mass were observed. 21
Fig. 6 Histology of B16/F10 cell implanted tumor tissue Tumor tissue were consisted with large spindle shaped cells with round nuclei and many mitotic figures(arrows) were seen. H-E stain, scale bar = 20㎛ 26
Fig. 7 Histology of B16/F10 cell implanted tumor tissue of control group. Massive necrosis(n) and infiltration of inflammatory cells(arrows) were seen at peripenia of tumor tissue(T). H-E stain, scale bar = 200㎛ 26
Fig. 8 Histology of B16/F10 cell implanted tumor tissue of Doxorubicin group Massive necrosis(n) and relatively thin area of alived tumor tissue(Tbetween arrows) were observed. H-E stain, scale bar = 400㎛ 27
Fig. 9 Histological structure of testis of normal group Well developed variable stages of seminiferous tubules. H-E stain, scale bar = 50㎛ 29
Fig. 10 Histological structure of testis of Doxorubicin group The structure of most seminiferous tubules were disrupted. Note the poor cellularity of seminferous epithelia and desquamated necrotic epthelia(arrows). Occasionally spermiogenesis were seen in some tububle (arrow head). H-E stain, scale bar = 50㎛ 29
Fig. 11 Histological structure of testis of Doxorubicin and Cultivated Wild Ginseng Herbal Acupuncture group. Note the dense cellularity of seminferous epithelia and active spermiogenesis(arrow heads) Compare with Fig. 10 H-E stain, scale bar = 50㎛ 30
Fig. 12 Histological structure of testis of Doxorubicin and Cultivated Wild Ginseng Herbal Acupuncture group. Most severe case of testotoxicity in this group. Cellularity of seminferous epithelia was increased than that of doxorubicn treated group. Compare with Fig. 10 H-E stain, scale bar = 50㎛ 30
Fig. 13 BrdU immunohistochemistry of testis of normal group Most of spermatogonia(arrow heads) were labelled strongly with BrdU. Ocassionally spermatocytes and spmatids (arrows) were also labelled with BrdU. BrdU immunohistochemical stain, scale bar = 30㎛ 33
Fig. 14 BrdU immunohistochemistry of testis of Doxorubicin group Any of spermatogonia labelled with BrdU were not observed. But weak labelling with BrdU was observed in some spermatocytes and sprematids (arrows). BrdU immunohistochemical stain, scale bar = 30㎛ 33
Fig. 15 BrdU immunohistochemistry of testis of Doxorubicin and Cultivated Wild Ginseng Herbal Acupuncture group Many spermatogoina(arrow heads) were labelled with BrdU. And the incidence and intensity of BrdU labelled spermatocytes and spermatids(arrows) were increased than those of doxorubicn group. Compare with Fig. 14. BrdU immunohistochemical stain, scale bar = 30㎛ 34
Fig. 16 BrdU immunohistochemistry of testis of Cultivated Wild Ginseng Herbal Acupuncture group Most of spermatogonia(arrow heads) were labelled strongly with BrdU. And the incidence and intensity of BrdU labelled spermatocytes and spermatids (arrows) were increased than those of normal group. BrdU immunohistochemical stain, scale bar = 30㎛ 34
Fig. 17 TUNEL immunohistochemistry of testis treated with Doxorubicin group A few of spermatogonia were labelled with TUNEL. TUNEL immunohistochemical stain, scale bar = 30㎛ 36
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