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논문명/저자명
Effects of Tang-gwi-eum-za-gagambang along with external spray therapy on the spontaneously occurring atopic dermatitis development in NC/Nga mouse / 김성훈
발행사항
나주 : 동신대학교 대학원, 2005.2
청구기호
TD 615.89 ㄱ699e
형태사항
ii, 66 p. ; 26 cm
자료실
서고(열람신청 후 1층 논문실)
제어번호
KDMT1200538919
주기사항
학위논문(박사) -- 동신대학교 대학원, 한의학, 2005.2
원문
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Ⅰ. Introduction 6

Ⅱ. Materials and methods 8

1. Materials 8

1) Reagents and apparatus 8

2) Experimental animals 9

3) Drugs 10

2. Procedure 12

1) Drug preparation 12

2) Drug administration into the NC/Nga atopy dermatitis mice 12

3) Clinical skin severity 13

4) Cytokine assay in the serum 13

5) Fluorescence Activated Cell Sorting analysis 13

6) Reverse Transcription Polymerase Chain Reaction 14

7) IL-6 and IL-13 quantitation in the skin tissues and cell surface molecule analysis by FACS 16

8) Histopathological examination 17

9) Lymphokine assay 17

10) Statistical analysis 17

Ⅲ. Results 19

1. Determination of AD severity in NC/Nga mice after drug administration 19

2. Effects of TG and ET on Serum IgE and IL-6 levels in AD mice 21

3. Effects of TG and ET on Serum IL-4 and IL-5 levels in NC/Nga mice 23

4. Effects of TG and ET on Serum IgE and IL-13 and IFN-γ levels in NC/Nga mice 24

5. Effects of TG and ET on spleen weight of NC/Nga mice 25

6. Effect of TG and ET on serum IgM in NC/Nga mice 26

7. Effects of TG and ET on serum IgG1 and IgG2a in NC/Nga mice 27

8. FACS analysis in the lymph node of NC/Nga mice 28

9. FACS analysis in the spleen 33

10. RT-PCR isolated from the facial skin tissues 39

1) Effects of TG and ET on IL-4 and IFN-γ mRNA expression 39

2) Effects of TG and ET on IL-5 and CCR3 mRNA expression 39

11. Effects of TG and ET on IL-6 and IL-13 mRNA expression 41

12. Determination of ratios of CD69+/CD3εand CD19+/CD44by FACS analysis 43

13. Histopathological analysis of lesioned ear skin tissues 44

14. Histopathological analysis of lesioned skin tissues 46

15. Lymphokine assay in the splenocytes 48

Ⅳ. Discussion 50

Ⅴ. Conclusion 59

References 61

Korean abstract 68

Table 1. Prescription of Tang-gwi-eum-za-gagambang (TG) 11

Table 2. Prescription of external spray treatment (ET) 11

Table 3. Cell Content in Lymph Node of NC/Nga Atopy

Dermatitis Model Mice 29

Table 4. Cell Content in Spleen of NC/Nga Atopy Dermatitis Model

Mice 34

Fig. 1. Clinical skin features and severity of dermatitis in NC/Nga mice 20

Fig. 2. Serum IgE and IL-6 elevation and development of

dermatitis in NC/Nga atopy dermatitis model mice 22

Fig. 3. Serum IL-4 and IL-5 elevation and development of

dermatitis in NC/Nga atopy dermatitis model mice 23

Fig. 4. Serum IL-13 and IFN-g elevation and development of

dermatitis in NC/Nga atopy dermatitis model mice 24

Fig. 5. Spleen weight in NC/Nga atopy dermatitis model mice 25

Fig. 6. IgM in NC/Nga atopy dermatitis model mice 26

Fig. 7. Immunoglobulin G1 and G2a in NC/Nga atopy dermatitis model

mice 27

Fig. 8. Effect of TG and ET cotreatment on CD3ε+ and CD19+ (%)

population in NC/Nga atopy dermatitis mouse lymph node

cells 30

Fig. 9. Effect of TG and ET cotreatment on CD4+ and CD8+ (%)

population in NC/Nga atopy dermatitis mice lymph node

cells 31

Fig. 10. Effect of TG and ET cotreatment on CD3ε+/ CD69+and

CD11a+/CD19+ (%) population in NC/ Nga atopy dermatitis

mice lymph node cells 32

Fig. 11. Effect of TG and ET cotreatment on CD3ε+ and CD19+ (%) population in NC/Nga atopy dermatitis mice spleen cells 36

Fig. 12. Effect of TG and ET cotreatment on CD4+ and CD8+ (%)

population in NC/Nga atopy dermatitis mice spleen cells 37

Fig. 13. Effect of TG and ET cotreatment on CD3ε+/CD69+ and

CD11a+/CD19+ (%) population in NC/Nga atopy dermatitis mice

spleen cells 38

Fig. 14. RT-PCR analysis for IL-4 and IFN-γ mRNA 40

Fig. 15. RT-PCR for IL-5 and CCR3 mRNA 40

Fig. 16. Culture supernatant IL-6 and IL-13 production in facial

skin biopsy of atopic dermatitis-like skin lesions in

NC/Nga mice 42

Fig. 17. FACS analysis of cultured facial & neck skin cells in

dermatitis-like skin lesions in NC/Nga mice 43

Fig. 18. Histologic features of ear lesion in NC/Nga mouse 45

Fig. 19. Histologic features of ear lesion in NC/Nga mouse 45

Fig. 20. Histologic features of lesioned skin tissue in NC/Nga mouse 47

Fig. 21. Histologic features of lesioned skin in NC/Nga mouse 47

Fig. 22. IL-4, IFN-γ synthesis in splenocytes from treated with TG

and ET in C57BL/6 mice 49

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