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영문요약 9
I. 서론 11
II. 연구방법 13
Cell Culture 13
Reagents 13
Flow Cytometric Analysis 14
Western Blot Analysis 14
Statistical Analysis 15
III. 결과 16
Viability of K562 cells after treatment with As₂O₃or ATRA 16
Induction of apoptosis by As₂O₃ 16
Erythroid differentiation of K562 cells 17
Expressions of Bcl-2, Bcl-XL, and Bax 17
IV. 고찰 19
V. 결론 23
참고문헌 24
국문요약 36
Figure 1. Viability of K562 cells after treatment with As₂O₃ or ATRA The percentage of viable K562 cells after treatment with 1 μM of As₂O₃ began to decrease significantly 9 days after treatment. The viabilities of K562 cells were 97%, 91%, and 74.5% by day 12 of treatment with 0.1 μM, 0.5 μM, and 1 μM of As₂O₃, respectively (*, p [ 0.05 , **, p [ 0.05). The viability of cells was not changed by adding 1 μM and 10 μM of ATRA until day 12. 31
Figure 2. Caspase 3 activation in K562 cells after treatment with As₂O₃ or ATRA. Activation of cleaved caspase-3 (17kDa) was observed in K562 cells treated with 1 μM of As₂O₃. Caspase-3 was not activated in K562 cells treated with a low dose As₂O₃ (0.1 μM and 0.5 μM) or ATRA (1 μM and 10 μM) 32
Figure 3A. Expression of glycophorin A in K562 cells after treatment with As₂O₃ or ATRA by using the flow cytometry and western blot analysis. A, Flow cytometric analysis showed that glycophorin A expression was increased in a dose dependent fashion in K562 cells after 3 days incubation with As₂O₃ but was unchanged in K562 cells incubated with ATRA. 33
Figure 3B. Expression of Bcl-2, Bcl-XL, and Bax in K562 cells after treatment with As₂O₃ or ATRA. The expression of Bax was reduced in a dose dependent fashion in K562 cells treated with As₂O₃ or ATRA. Bcl-XL was increased in a dose dependent fashion in K 562 cells treated with As₂O₃ whereas Bcl-XL remained unaltered when treated with ATRA. 34
Figure 4. Expression of Bcl-2, Bcl-XL, and Bax in K562 cells after treatment with As₂O₃or ATRA. The expression of Bax was reduced in a dose dependent fashion in K562 cells treated with As2O3 or ATRA. Bcl-XL was increased in a dose dependent fashion in K562 cells treated with As₂O₃whereas Bcl-XL remained unaltered when treated with ATRA. The expression of Bcl-2 was increa 35
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