본문 바로가기 주메뉴 바로가기
22대 대한민국 국회 국회정보길라잡이 국회의원검색
회원가입 로그인
HOME

정보검색

소장정보 검색

국회도서관 홈으로 정보검색 소장정보 검색
결과 내 검색 동의어 포함
결과 내 검색 동의어 포함

-

목차보기

표제지

목차

영문요약 9

I. 서론 11

II. 연구방법 13

Cell Culture 13

Reagents 13

Flow Cytometric Analysis 14

Western Blot Analysis 14

Statistical Analysis 15

III. 결과 16

Viability of K562 cells after treatment with As₂O₃or ATRA 16

Induction of apoptosis by As₂O₃ 16

Erythroid differentiation of K562 cells 17

Expressions of Bcl-2, Bcl-XL, and Bax 17

IV. 고찰 19

V. 결론 23

참고문헌 24

국문요약 36

그림목차

Figure 1. Viability of K562 cells after treatment with As₂O₃ or ATRA The percentage of viable K562 cells after treatment with 1 μM of As₂O₃ began to decrease significantly 9 days after treatment. The viabilities of K562 cells were 97%, 91%, and 74.5% by day 12 of treatment with 0.1 μM, 0.5 μM, and 1 μM of As₂O₃, respectively (*, p [ 0.05 , **, p [ 0.05). The viability of cells was not changed by adding 1 μM and 10 μM of ATRA until day 12. 31

Figure 2. Caspase 3 activation in K562 cells after treatment with As₂O₃ or ATRA. Activation of cleaved caspase-3 (17kDa) was observed in K562 cells treated with 1 μM of As₂O₃. Caspase-3 was not activated in K562 cells treated with a low dose As₂O₃ (0.1 μM and 0.5 μM) or ATRA (1 μM and 10 μM) 32

Figure 3A. Expression of glycophorin A in K562 cells after treatment with As₂O₃ or ATRA by using the flow cytometry and western blot analysis. A, Flow cytometric analysis showed that glycophorin A expression was increased in a dose dependent fashion in K562 cells after 3 days incubation with As₂O₃ but was unchanged in K562 cells incubated with ATRA. 33

Figure 3B. Expression of Bcl-2, Bcl-XL, and Bax in K562 cells after treatment with As₂O₃ or ATRA. The expression of Bax was reduced in a dose dependent fashion in K562 cells treated with As₂O₃ or ATRA. Bcl-XL was increased in a dose dependent fashion in K 562 cells treated with As₂O₃ whereas Bcl-XL remained unaltered when treated with ATRA. 34

Figure 4. Expression of Bcl-2, Bcl-XL, and Bax in K562 cells after treatment with As₂O₃or ATRA. The expression of Bax was reduced in a dose dependent fashion in K562 cells treated with As2O3 or ATRA. Bcl-XL was increased in a dose dependent fashion in K562 cells treated with As₂O₃whereas Bcl-XL remained unaltered when treated with ATRA. The expression of Bcl-2 was increa 35

추천서가 (다양한 추천 자료를 만나보세요)

권호기사보기

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 기사목차