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국회도서관 홈으로 정보검색 소장정보 검색

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I. Introduction 5

II. Materials and methods 8

1. Reagents 8

2. Preperation of herbal-acupuncture solution 8

3. Cell culture 8

4. MTT cytotoxicity assay 9

5. DAPI staining 9

6. TUNEL assay 10

7. RT-PCR analysis 10

8. Caspase-3 activity assay 11

9. Statistical analysis 12

III. Results 13

1. Effect of HSCJ on SNU-668 cells cytotoxicity 13

2. Morphological changes induced by HSCJ 15

3. Effect of HSCJ on mRNA expression of bax, bcl-2 and caspase-3 17

4. Caspase-3 enzyme activity analysis 19

IV. Discussion 21

V. Conclusion 24

References 25

Korean abstract 30

Abstract 31

표목차

Fig. 1. Cytotoxic effects of HSCJ 14

Fig. 2. Characterization of HSCJ-induced cell death in

SNU-668 cells 16

Fig. 3. Results of RT-PCR analysis of the mRNA of bax,

bcl-2 and caspase-3 18

Fig. 4. Result of caspase-3 enzymeactivity assay 20