Contents 1
Comparative analysis of yeast cell viability at exponential and stationary growth phases / Yejin An ; Nayoon Jo ; Hyeji Kim ; Dahye Nam ; Woorim Son ; Jinkyu Park 1
Abstract 1
1. Introduction 1
2. Experimental 2
2.1. Cell strain and reagents 2
2.2. Yeast cell culture 2
2.3. Direct cell counting and methylene blue (MB) assay 2
2.4. Single plate-serial dilution spotting (SP-SDS) assay 3
2.5. Cell counting kit (CCK)-8 assay 3
2.6. Statistical analysis 3
3. Results and Discussion 3
3.1. Cell growth monitoring : Optical density and direct cell counting 3
3.2. Proliferative capacity : SP-SDS assay 5
3.3. Metabolic activity : MB and CCK-8 assays 6
4. Conclusions 7
References 7