Contents
Liquid chromatography-tandem mass spectrometric analysis of oleracone D and its application to pharmacokinetic study in mice / Dong Yu Lim ; Tae Yeon Lee ; Jaehyeok Lee ; Im-Sook Song ; Young Taek Han ; Min-Koo Choi 1
Abstract 1
1. Introduction 1
2. Experimental 2
2.1. Chemicals and reagents 2
2.2. Preparation of the stock and working solutions 2
2.3. Preparation of calibration standards and quality control samples 3
2.4. Sample preparation 3
2.5. Instalment conditions 3
2.6. Method validations 3
2.7. Pharmacokinetic study 4
2.8. Plasma protein binding 4
2.9. Plasma stability 5
2.10. Data analysis and statistics 5
3. Results and Discussion 5
3.1. LC-MS/MS analysis of oleracone D 5
3.2. Selectivity and linearity 5
3.3. Precision and accuracy 6
3.4. Matrix effect and recovery 7
3.5. Stability 7
3.6. Pharmacokinetics of oleracone D 7
4. Conclusions 9
References 9
Authors' Position 9