Contents
Effective determination of nicotine enantiomers from e-liquids and biological fluids by high performance liquid chromatography (HPLC) using dispersive liquid-liquid microextraction (DLLME) / Seunghoon Song ; Seung-Woon Myung 1
Abstract 1
1. Introduction 1
2. Experimental 2
2.1. Reagents and materials 2
2.2. Real sample analysis 3
2.3. Apparatus and instrumentation 3
2.4. Sample preparation 3
3. Results and Discussion 4
3.1. Comparison of GC and HPLC methods 4
3.2. Parameter optimization 5
3.3. Effect of sample pH 5
3.4. Effect of the combined extraction and dispersion solvents 6
3.5. Effect of volume ratio of dspersion solvent and extraction solvent 6
3.6. Effect of total volume of dispersion and extraction solvents 6
3.7. Effect of salting-out 7
3.8. Effect of laboratory temperature 8
3.9. Method Validation 8
3.10. Assay of real samples 9
4. Conclusions 10
References 11
Authors' Position 11