Contents
Simultaneous qualitative and quantitative analysis of morroniside and hederacoside D in extract mixture of Cornus officinalis and Stauntonia hexaphylla leaves to improve benign prostatic hyperplasia by HPLC-UV / Gao Dan ; Chong Woon Cho ; Le Ba Vinh ; Jin Hyeok Kim ; Kyoung Won Cho ; Young Ho Kim ; Jong Seong Kang 1
Abstract 1
1. Introduction 1
2. Experimental 3
2.1. Chemical and reagents 3
2.2. Preparation of CS extract 3
2.3. Apparatus and chromatographic condition 3
2.4. Sample and standard preparation 3
2.5. Method validation 3
3. Results and Discussion 4
3.1. Selection of marker compounds and optimization of the chromatographic condition 4
3.2. HPLC-ESI-MS/MS analysis of marker compounds 4
3.3. Validation of the method 5
3.4. Comparison of hederacoside D in S. hexaphylla leaves from different harvest times 6
3.5. Application to the analysis of 20 batches of CS extracts 7
4. Conclusions 7
References 7