Contents
The impact of freeze-drying on the glycoproteomic profiles of human milk / Won-Ho Hahn ; Seong-Phil Bae ; Hookeun Lee ; Jong-Moon Park ; Suyeon Park ; Joohyun Lee ; Nam Mi Kang 1
Abstract 1
1. Introduction 1
2. Methods and Materials 2
2.1. Human Milk samples 2
2.2. Chemicals and reagents 2
2.3. Protein assays 3
2.4. Automated digestion of HM proteins 3
2.5. Automated enrichment of glycopeptide 3
2.6. Liquid chromatography mass spectrometry(LC-MS/MS) analysis 3
2.7. Peptide identification and protein quantification 4
2.8. Statistical and bioinformatics analysis 4
3. Results 5
3.1. Comparisons of the human milks glycoproteins between the different lactation stage groups (15 and 60 postpartum days) and freeze-drying status (pre- and post freeze-drying) 5
3.2. Partial correlation analysis of the human milks glycoproteins between the different lactation stage groups (15 and 60th lactation day) and freeze-drying status (pre- and post freeze-drying) 5
3.3. Gene ontology analysis 5
4. Discussion 6
References 8