Title Page
Contents
ABSTRACT 8
INTRODUCTION 9
MATERIALS AND METHODS 12
2.1. Preparation of cell culture and hypoxic condition 12
2.2. Preparation of alginate-gelatin hydrogel microsphere 12
2.3. Measurement of cell viability and proliferation 13
2.4. In vitro NK cell-mediated cytotoxicity assay 14
2.5. Cell viability and cytotoxicity under hypoxic condition 15
2.6. Gene expression analysis (RT-qPCR) 15
2.7. Statistical analysis 16
RESULTS 18
3.1. Physical characteristics of alginate-gelatin hydrogel microsphere generated by electrospray 18
3.2. Effect of electrospray condition on NK cell viability 18
3.3. Proliferation of encapsulated NK cells with hydrogel microsphere 22
3.4. NK cell-mediated tumor-killing effect of encapsulated NK cells with hydrogel microsphere 24
3.5. Cell viability and HIF-1α expression of encapsulated NK cells with hydrogel microsphere under hypoxic condition 29
3.6. NK cell-mediated tumor-killing effect of encapsulated NK cells with hydrogel microsphere under hypoxic condition 32
DISCUSSION 35
CONCLUSION 37
REFERENCES 39
Table 1. Primer sequences of RT-qPCR 17
Figure 1. Schematic illustration of KHYG-1 cells laden alginate-gelatin hydrogel microspheres generated by electrospray technology for cancer immunotherapy under hypoxia... 11
Figure 2. Generation of alginate-gelatin hydrogel microsphere using electrospray. 21
Figure 3. Proliferation of NK cells within alginate-gelatin hydrogel microsphere. 23
Figure 4. NK cell-mediated tumor-killing effect measurement of encapsulated KHYG-1 cells with hydrogel microsphere under hypoxic condition. 28
Figure 5. Induction of hypoxic condition and cell viability measurement. 31
Figure 6. Evaluation of NK cell-mediated tumor-killing effect under hypoxic condition. 34