Title Page
Contents
Part Ⅰ. Fluorescent detection of inorganic mercury and methylmercury in aqueous solution with micelles and live cells using fluorescent chemosensors based on Trp amino acid and cycloalkylamine-7- sulfonyl-2,1,3-benzoxadiazole 9
Abstract 10
요약 11
Introduction 12
1. Experimental 16
1.1. Materials and reagents 16
1.2. Solid phase synthesis 17
1.3. UV/Vis and fluorescence measurements 21
1.4. Dissociation constant determination 22
1.5. Determination of detection limit (LOD), and quantum yield 23
1.6. Cell viability and detection of CH₃Hg⁺ and Hg²⁺ in cells by confocal microscopy 24
2. Results and discussion 25
2.1. Design of ABD-based amino acid probe for Hg²⁺ and CH₃Hg⁺ 25
2.2. Fluorescence response of chemosensors to Hg²⁺ and CH₃Hg⁺ in aqueous buffered solutions 26
2.3. Fluorescence response of chemosensors to Hg²⁺ and CH₃Hg⁺ in micelle system 29
2.4. Job's plot analysis of 5 for Hg²⁺ and CH₃Hg⁺ 33
2.5. Binding affinity of chemosensors with Hg²⁺ and CH₃Hg⁺ 34
2.6. Comparison of chemosensors 1-5 for quantum yield, binding affinity and fluorescence change in PC micelles 37
2.7. Metal selectivity study of chemosensors 1 and 5 39
2.8. Effect of pH on the fluorescence response of 5 for Hg²⁺ and CH₃Hg⁺ 43
2.9. Fluorescence response of chemosensors to Hg²⁺ and CH₃Hg⁺ at pH 10 45
2.10. Detection limit of 5 with Hg²⁺ and CH₃Hg⁺ in PC micelles 47
2.11. Binding mode study of 5 for Hg²⁺ and CH₃Hg⁺ 49
2.12. Reversible test for Hg²⁺ and CH₃Hg⁺ using EDTA and GSH 53
2.13. Cell images of 5 with Hg²⁺ and CH₃Hg⁺ 56
3. Conclusions 60
4. Characterization 61
Reference 74
Part Ⅱ. Ratiometric sensing for Hg²⁺ and CH₃Hg⁺ ions in aqueous solution and live cells using fluorescent probes based on PheHis 80
Abstract 81
요약 82
Introduction 83
1. Experimental 87
1.1. Materials and reagents 87
1.2. Solid phase synthesis 88
1.3. UV/Vis and fluorescence measurements 90
1.4. Determination of dissociation constant and LOD 91
1.5. Dynamic light scattering (DLS) and circular dichroism (CD) measurements 92
1.6. Fibril formation analysis using CLSM and TEM 93
1.7. Cell viability and detection of CH₃Hg(I) and Hg(II) in cells 94
2. Results and discussion 95
2.1. Design of excimer emission based self-assembly dipeptide probe for Hg(II) and CH₃Hg(I) 95
2.2. Fluorescence response of probes to Hg(II) and CH₃Hg(I) 96
2.3. Selective and interference test of metal ions 99
2.4. Study on reducing Cu(II) interference of 1 for Hg(II) and CH₃Hg(I) 101
2.5. Time dependent fluorescence response of 1 103
2.6. Effect of pH on the fluorescence response of 1 for Hg(II) and CH₃Hg(I) 105
2.7. Binding mode study of 1 with Hg(II) and CH₃Hg(I) 107
2.8. Job's plot analysis and dissociation constant of 1 112
2.9. Reversible test of 1 for Hg(II) and CH₃Hg(I) 115
2.10. Fibril formation of 1 with Hg(II) 118
2.11. Application of 1 for detecting Hg(II) and CH₃Hg(I) in cells 122
2.12. Detection limit of 1 for Hg(II) and CH₃Hg(I) 124
3. Conclusions 126
4. Characterization 127
Reference 132