Title Page
Contents
Korean Abstract 9
Abstract 11
1. Introduction 13
2. Materials and Methods 16
2.1. Construction of chimeric FGF21/HGFR receptor 16
2.2. Cell culture and Treatment 17
2.3. Periodic acid-schiff (PAS) staining 18
2.4. LDL uptake assay 19
2.5. Reverse transcription (RT)-PCR and real-time PCR 20
2.6. Statistical analysis 21
3. Results 22
3.1. Construction of Stable AML12 Cell Line for Expression of chimeric FGF21/HGFR 22
3.2. FGF21/HGFR on AML12 cells are closely related glycogen accumulation ability. 24
3.3. FGF21/HGFR promotes LDL uptake on AML12 cells 26
3.4. Evaluation of the expression for hepatocyte function-related genes 28
4. Discussion 31
5. Conclusion 34
References 35
Table 1. Sequence of primer used in the real-time PCR. 21
Figure 1. Construction of chimeric FGF21/HGFR receptor. 23
Figure 2. Glycogen accumulation capacity was increased in chimeric FGF21/HGFR on AML12. 25
Figure 3. LDL uptake was upregulated in chimeric FGF21/HGFR on AML12. 27
Figure 4. In AML12 where steatosis was induced by palmitate, chimeric FGF21/HGFR showed an indicator of liver regeneration. 29