Title Page
Contents
Abstract 9
Ⅰ. INTRODUCTION 11
Ⅱ. MATERIALS AND METHODS 14
1. Materials 14
2. Preparation of multifunctional PLLA films 14
2.1. The first coating Layer: TIBA/MH coating on PLLA films 14
2.2. The second coating layer: PDA/Cys coating on PLLA films 15
3. Characterization of coated PLLA films 15
3.1. X-Ray images of radiopaque PLLA films 15
3.2. Quantitative analysis of MH 16
3.3. Water contact angle 16
3.4. NO release analysis 16
3.5. Elemental composition and chemical bonding analysis 17
3.6. Surface morphology 17
3.7. Degradation behavior 18
4. In vitro study 18
4.1. Cell culture 18
4.2. Cell proliferation assay 19
4.3. Inflammation response analysis 19
4.4. Platelet adhesion test 20
5. Statistical analysis 20
Ⅲ. RESULTS 21
1. Preparation of radiopaque and NO released film 21
2. Characterization of radiopaque films 23
3. Characterization of MH and NO released films 25
4. Surface characterization of coated films 28
5. Degradation behavior of the films and cumulative release of TIBA 32
6. In vitro cell proliferation 35
7. Inflammation assay 40
8. Platelet adhesion and activation 42
Ⅳ. Discussion 45
Ⅴ. CONCLUSION 48
REFERENCES 49
ABSTRACT IN KOREAN 55
Table 1. Surface characterization of coated PLLA films 29
Figure 1. X-ray images of PLLA films coated with the various concentration of TIBA 24
Figure 2. Characterization of MH released film 26
Figure 3. Characterization of NO released film 27
Figure 4. ATR-FTIR and XPS spectra of each coated PLLA films 30
Figure 5. Surface morphology of each coated PLLA films. 31
Figure 6. Degradation Behavior of the Films 33
Figure 7. Cumulative release profile of TIBA 34
Figure 8. In vitro cell proliferation assay 37
Figure 9. In vitro cell proliferation assay 39
Figure 10. The expression levels of cytokine factor 41
Figure 11. Platelet adhesion and activation 44
Scheme 1. Schematics for multilayer functionalized drug-eluting balloon. 22