표제지
국문초록
목차
Ⅰ. 서론 14
Ⅱ. 재료 및 방법 19
1. 실험 재료 19
1.1. C. fusca 및 딸기 재배조건 19
1.2. 실험 시약 20
1.3. 실험 장비 20
2. 실험 전처리 21
2.1. C. fusca 전처리 21
2.2. 딸기 전처리 21
3. 생리활성 평가 22
3.1. DPPH 라디칼 소거능 22
3.2. ABTS⁺ 라디칼 소거능 22
3.3. DNA 보호 효능 23
3.4. 티로시나아제 저해 효능 23
3.5. 엘라스타아제 저해 효능 24
4. UPLC-Q-TOF-MS 및 HPLC 분석조건 25
4.1. 대사체 구조분석 조건 25
4.2. 안토시아닌 및 당 분석조건 26
Ⅲ. 결과 및 고찰 27
1. C. fusca 생리활성 평가 27
1.1. 항산화 효능 27
1.2. 미백 및 항노화 특성 33
2. 대사체 분석 37
2.1. 음이온 모드 분석 37
2.2. 양이온 모드 분석 49
3. 수확 시기별 C. fusca 무처리 및 처리 딸기 유용성분 분석 67
3.1. 대사체 분석 67
3.2. 안토시아닌 분석 79
3.3. 당 분석 83
Ⅳ. 결론 86
참고문헌 88
부록 94
Abstract 141
Table 1. Conditions of UPLC-Q-TOF-MS. 25
Table 2. Conditions of HPLC. 26
Table 3. Identification of metabolite profiles in the methanol extract of C. fusca by the negative ion mode of UPLC-Q-TOF-MS analysis. 45
Table 4. Identification of metabolite profiles in the methanol extract of C. fusca by the positive ion mode of UPLC-Q-TOF-MS analysis. 63
Table 5. Identification of metabolite profiles of strawberry by the negative ion mode of UPLC-Q-TOF-MS analysis. 78
Figure 1. (A) The cultured C. fusca sample. (B) Various harvest periods (April 1, April 6, April 8, April 13). 19
Figure 2. DPPH radical scavenging activities in the methanol extract of C. fusca. 28
Figure 3. ABTS radical scavenging activities in the methanol extract of C. fusca. 30
Figure 4. DNA protectant activities in the methanol extract of C. fusca. lane 1, pUC18 only; lane 2, pUC18 with DDT only;... 32
Figure 5. Tyrosinase inhibitory effects in the methanol extract of C. fusca. 34
Figure 6. Elastase inhibitory effects in the methanol extract of C. fusca. 36
Figure 7. UPLC-Q-TOF-MS chromatogram using negative ion mode. 37
Figure 8. MS and MS² fragment ion patterns of identified peak 1. 38
Figure 9. MS and MS² fragment ion patterns of identified peak 2. 39
Figure 10. MS and MS² fragment ion patterns of identified peak 3. 40
Figure 11. MS and MS² fragment ion patterns of identified peak 5. 41
Figure 12. MS and MS² fragment ion patterns of identified peak 13. 42
Figure 13. Metabolite structures through negative ion mode of UPLC-Q-TOF-MS. 44
Figure 14. Mass fragmentation patterns of 26 metabolite compositions using negative ion mode of UPLC-Q-TOF-MS analysis. 48
Figure 15. UPLC-Q-TOF-MS chromatogram using positive ion mode. 49
Figure 16. MS and MS² fragment ion patterns of identified peak 1. 50
Figure 17. MS and MS² fragment ion patterns of identified peak 3. 51
Figure 18. MS and MS² fragment ion patterns of identified peak 4. 52
Figure 19. MS and MS² fragment ion patterns of identified peak 5. 53
Figure 20. MS and MS² fragment ion patterns of identified peak 6. 54
Figure 21. MS and MS² fragment ion patterns of identified peak 7. 55
Figure 22. MS and MS² fragment ion patterns of identified peak 11. 56
Figure 23. MS and MS² fragment ion patterns of identified peak 25. 57
Figure 24. MS and MS² fragment ion patterns of identified peak 30. 58
Figure 25. MS and MS² fragment ion patterns of identified peak 33. 59
Figure 26. MS and MS² fragment ion patterns of identified peak 35. 60
Figure 27. Metabolite structures through positive ion mode of UPLC-Q-TOF-MS. 62
Figure 28. Mass fragmentation patterns of 26 metabolite compositions using negative ion mode of UPLC-Q-TOF-MS analysis. 66
Figure 29. UPLC-Q-TOF-MS chromatogram using negative ion mode. 68
Figure 30. MS and MS² fragment ion patterns of identified peak 1. 69
Figure 31. MS and MS² fragment ion patterns of identified peak 2. 70
Figure 32. MS and MS² fragment ion patterns of identified peak 3. 71
Figure 33. MS and MS² fragment ion patterns of identified peak 4. 72
Figure 34. MS and MS² fragment ion patterns of identified peak 5. 73
Figure 35. MS and MS² fragment ion patterns of identified peak 6. 74
Figure 36. MS and MS² fragment ion patterns of identified peak 7. 75
Figure 37. Metabolite structures through negative ion mode of UPLC-Q-TOF-MS. 77
Figure 38. Fruits were selected in the 80-90% colorations and weights of 20 ± 5 g during the different harvest... 79
Figure 39. Changes in sugar contents of strawberry on April 1 after C. fusca treatment. 81
Figure 40. Changes in sugar contents of strawberry on April 6 after C. fusca treatment. 81
Figure 41. Changes in sugar contents of strawberry on April 8 after C. fusca treatment. 82
Figure 42. Changes in sugar contents of strawberry on April 13 after C. fusca treatment. 82
Figure 43. HPLC chromatograms and chemical structures of standard samples: (1) fructose (2) glucose (3) sucrose (4) maltose (5) lactose. 83
Figure 44. Changes in sugar contents after C. fusca treatment. (A) April 1, (B) April 6 85
Figure 45. Changes in sugar contents after C. fusca treatment. (A) April 8, (B) April 13 85