Title Page
국문초록
Contents
Ⅰ. Introduction 10
Ⅱ. Materials and Methods 12
1. Preparation of Samples 12
2. Physicochemical Characteristics of Samples 14
1) Physical Characteristics of Samples 14
2) Surface Characteristics of Samples 17
3) Chemical Characteristics of Samples 19
3. Biological Characteristics of Samples 20
1) Primary culture of Stem cells obtained from human exfoliated deciduous teeth (SHED) 20
2) Cell viability 22
3) Cell Differentiation 23
4. Antibacterial Characteristics 24
5. Statistical Analysis 26
Ⅲ. Results 27
1. Physicochemical Characteristics 27
1) Physical Characteristics of Samples 27
2) Surface Characteristics of Samples 32
3) Chemical Characteristics of Samples 35
2. Biological Characteristics 38
(1) Cell viability 38
(2) Odontoblast differentiation 40
3. Antibacterial Characteristics 42
Ⅳ. Discussion 44
Ⅴ. Conclusion 58
References 60
Abstract 69
Table 1. Manufacturer and composition of the materials 13
Table 2. Setting time, solubility, and compressive strength of the tested materials 29
Table 3. Flow, film thickness of the tested materials 30
Table 4. Elemental composition of the tested materials 33
Table 5. Ion release concentration of elements (ppm=mg/L) (mean ± SD). 36
Fig. 1. Schematic Representation of physical properties (setting time, solubility,compressive strength, flow, and film thickness). 31
Fig. 2. Schematic Representation and surface analysis using SEM, XRD, and EDS. 34
Fig. 3. Schematic Representation of ICP-AES and pH value of four CSCs. 37
Fig. 4. Schematic representation of the initial cell viability of SHED after exposure to four CSCs. 39
Fig. 5. Schematic of experiments and results of odontoblastic differentiated SHED exposed to diluted CSCs. 41
Fig. 6. Schematic representation of antibacterial effects using four CSCs analyzed with E. faecalis, 10⁶ CFUs/mL. 43