Title Page
ABSTRACT
Contents
1. Introduction 10
2. Materials and methods 12
2.1. Antibodies and reagents 12
2.2. Cells 12
2.3. Animals 13
2.4. T cell activation 13
2.5. Confocal microscopy 14
2.6. Scanning Electron microscopy 15
2.7. Analysis of TMPs and EVs 15
2.8. Tumor Exosomes (TEX) 16
2.9. Flow cytometry analysis 17
2.10. Measurement of surface proteins on DCs 17
2.11. Cell transfection and viral infection 18
2.12. Statistics 18
3. Results 19
3.1. TISs are quickly released from effector T cell microvilli during the antigen recognition 19
3.2. TISs recruit immune cells via DC activation both in vitro and in vivo 20
3.3. Pre-treatment with TISs in skin dermis prevents B16 melanoma metastasis through immune response inducing in vivo 21
4. Discussion 29
5. Abstract in Korean 31
6. References 33
Fig. 1. TISs are quickly released from T cell microvilli on APC. 24
Fig. 2. TISs recruit immune cells through DC activation. 26
Fig. 3. Administration of TISs in the dermis of the skin induces the anti-cancer effect 28