Title Page
Abstract
Contents
Ⅰ. INTRODUCTION 10
Ⅱ. MATERIALS AND METHODS 12
1. Chemicals 12
2. Cell culture 12
3. Cell based fluorescence assay and data anaylsis 13
4. Injection of synthesized cRNA in Xenopus Oocytes 13
5. Electrophysiologic recordings and data analysis 14
Ⅲ. RESULTS 17
3.1. Screening through a cell based fluorescence assay 17
3.2. Dose-dependent activation of DBTB-1 and inhibition by paxilline 18
3.3. Macrosocpic recording and gating kinetics after treating DBTB-1 18
3.4. Single channel recording after treating DBTB-1 19
Ⅳ. DISCUSSION 35
Ⅴ. APPENDIX 37
Ⅵ. 국문 초록 38
Ⅶ. REFERENCES 39
Table 1. Structure activity of classified BKCa channel modulators through in vitro assay[이미지참조] 21
Figure 1. Activity of BKCa channel modulators through cell-based fluorescence assay[이미지참조] 23
Figure 2. Dose-dependent increase and apparent half-maximal effective concentration of DBTB-1 25
Figure 3. Completely inhibition by paxilline of fluorescence signal 27
Figure 4. The tail currents induced by DBTB-1 showed a dose-dependent increase. 29
Figure 5. Effects of DBTB-1 on activation and deactivation of macroscopic currents. 31
Figure 6. Long opening of BKCa channels induced by DBTB-1[이미지참조] 33
Supplementary Figure 1. Effects of BKCa channel activators, DBTB-1, CTIBD, NS11021, NS1619 and Rottlerin.[이미지참조] 37