Title Page
ABSTRACT
Contents
1. INTRODUCTION 8
2. MATERIALS AND METHODS 9
2.1. Yeast genomic DNA prep 9
2.2. Yeast transformation 9
2.3. Rapamycin treatment and fluorescence microscopy 10
3. RESULTS 11
3.1. Construction of yeast strains with single or double deleted genes and confirmation 11
3.2. sey1△ and lnp1△ strains decrease PMN vesicles 11
3.3. yop1△ strain does not change PMN vesicles, but rtn1△ strain reduces PMN vesicles 12
3.4. lnp1△sey1△ strain reduce PMN vesicle formation similarly to sey1△ and lnp1△ strains 12
4. DISCUSSION AND CONCLUSION 22
5. REFERENCES 23
6. ABSTRACT IN KOREAN 24
Table 1. Yeast strains used in this study 13
Figure 1. Identification of genes and proteins removed from yeast strain 15
Figure 2. Decreased number of PMN vesicles by sey1△ and lnp1△ strains 17
Figure 3. Decreased number of PMN vesicles by rtn1△ 19
Figure 4. Decreased number of PMN vesicles by sey1△lnp1△ 21