Title Page
Abstract
Contents
Introduction 8
Materials and methods 10
1. Cells, plasmids, and reagents 10
2. In vitro migration, invasion, colony-forming, and proliferation assay 10
3. Western blot analysis 11
4. RNA extraction and quantitative polymerase chain reaction 11
5. Luciferase reporter gene assay 12
6. Chromatin immunoprecipitation assay 12
7. Animal studies 12
8. Immunohistochemistry analysis 13
9. Analysis of dataset for human samples and cell lines 13
10. mRNA-sequencing analysis 14
11. Statistical analysis 14
12. Availability of data and materials 15
Results 17
Discussion 44
References 46
Abstract (in Korean) 50
Fig. 1. ZBTB7A expression was decreased in GBM patients 18
Fig. 2. ZBTB7A expression was decreased in GBM cells 19
Fig. 3. ZBTB7A-knockdown is associated with cell motility and the movement of cellular components 21
Fig. 4. ZBTB7A-knockdown promotes cell proliferation, migration, invasion, and colony formation in U87 cells 22
Fig. 5. ZBTB7A-overexpressing inhibits cell proliferation, migration, invasion, and colony formation in LN229 and U343 cells 24
Fig. 6. EPB41L5 is the target gene of ZBTB7A in GBM cells 26
Fig. 7. EPB41L5 inversely correlates with ZBTB7A in patients 27
Fig. 8. ZBTB7A negatively regulates EPB41L5 in GBM cells and patients 29
Fig. 9. ZBTB7A-knockdown induces EMT process 30
Fig. 10. ZBTB7A inhibits transcriptional activity of EPB41L5 32
Fig. 11. ZBTB7A binds specifically to the EPB41L5 promoter 34
Fig. 12. Mutation of ZBTB7A rescues the transcriptional activity of EPB41L5 36
Fig. 13. ZBTB7A negatively regulates the transcriptional activity of EPB41L5 38
Fig. 14. ZBTB7A-overexpressing inhibits tumorigenesis 40
Fig. 15. ZBTB7A-knockdown promotes EMT and tumorigenesis 42
Fig. 16. Graphical abstract 43