Title Page
Abstract
Contents
1. Introduction 8
2. Materials and Methods 11
2.1. Patients and Samples collection for proteome analysis 11
2.2. Urinary sample preparation and proteome analysis 11
2.3. Mice 12
2.4. Enzyme-Linked Immunosorbent assays (ELISA) 12
2.5. Histologic analysis 14
2.6. Immunofluorescence staining 15
2.7. Statistical analysis 15
3. Results 16
3.1. ORM1, SERPINC1 and Transferrin were more expressed in initial LN than SLE. 16
3.2. ORM1 and SERPINC1 were increased in early-stage urine of MRL/lpr mice, but not Transferrin. 21
3.3. Histologic analysis of MRL/lpr mice 24
4. Discussion 27
5. Conclusion 30
6. References 31
국문요약 34
Table 1. Correlation between potential urine biomarkers and activity/chronicity indexes. 26
Figure 1. Quantitative urinary proteome analysis. 17
Figure 2. Comparative urinary proteome analysis between the iLN and SLE group. 20
Figure 3. The levels of anti-dsDNA autoantibody in mice. 21
Figure 4. The levels of biomarker candidates and albumin in urine of mice. 23
Figure 5. Histologic analysis of kidney at 23 weeks age of mice. 25