Title Page
Contents
Abstract 10
Introduction 13
Materials and Methods 17
1. Human HI or PB γδ T cell Expansion 17
2. Cell Culture 19
3. Pyrophosphate Assay 19
4. Flow Cytometry 20
5. Degranulation Assay 21
6. In Vivo Experiment 22
7. Lactate Dehydrogenase (LDH) Assay 23
8. Statistics 24
Results 25
1. HI γδ T Cells Exhibited Strong Cytotoxicity against HCC cell Lines 25
2. HI γδ T Cells and PB γδ T Cells Differentially Expressed an Array of Activating and Inhibitory Receptors 29
3. Cytotoxicity of HI γδ T Cells Against SNU398 Cells Rely on NKG2D Expression 38
4. Activating and Death Ligand Signaling Are Associated with Cytotoxicity of HI γδ T Cells against Prostate Cancer Cells 43
Discussion 50
References 53
국문요약 60
Figure 1. HI γδ T Cells Effectively Eradicated HCC Cell Lines. 27
Figure 2. Gating Strategy for In Vivo Experiment. 28
Figure 3. Percentages of Activating and Inhibitory Receptors, and Death Ligands Expressed in HI γδ T Cells and PB γδ T Cells. 32
Figure 4. MFI of Activating and Inhibitory Receptors, and Death Ligands Expressed in HI γδ T Cells and PB γδ T Cells. 34
Figure 5. Gating Strategies and Representative Flow Cytometric Plots of Cell Surface Markers on HI Vδ1 and Vδ2 γδ T Cells. 36
Figure 6. Improved Proliferation of HI γδ T Cells in the Presence of Anti-HVEM Peptide and Anti-PD-L1 mAb. 37
Figure 7. Expression of DNAM-1 Ligands in HCC cell lines. 39
Figure 8. NKG2D Expression Contributes to Cytotoxicity of HI γδ T Cells against SNU398 Cells. 40
Figure 9. HI γδ T Cells Degranulates More in Response to Huh7 Cells Than to SNU398 Cells. 42
Figure 10. Cytotoxicity of HI γδ T cells Correlates to Activating, and Death Ligand Signaling in Prostate Cancer Cell Lines. 46
Figure 11. Representative Flow Cytometric Plots of Cell Surface Markers on the Prostate Cancer Cells, PC3, Du145 and LNCaP. 49