Title Page
Abstract
Contents
Introduction 12
Material and Methods 15
Characterization of MSCs 15
Cell culture 15
Optical diffraction tomography (ODT) imaging 16
Adipogenesis and osteogenesis of LD-MSCs in vitro 16
Total RNA extraction and quantitative RT-PCR 17
Cell cycle analysis 20
Statistical analysis 20
Results 21
Characterization of Mesenchymal Stem Cells Derived from Different Tissues 21
Cell properties alteration of senescent LD-MSCs 25
Time-dependent differences in miRNA expression levels 34
TGF-β1 treatment induces cellular activity 39
Discussion 45
Conclusion 48
References 49
국문요약 52
Abbreviation 53
Table 1. Primer information 18
Figure 1. Comparison of the characteristics between human liver-derived stem cells (LD-MSCs) and Wharton's jelly -derived mesenchymal stem cells (WJ-MSCs). 24
Figure 2. Morphological changes and measurement of differentiation capacity-related genes in senescent LD-MSCs. 28
Figure 3. Cell growth rate reduction and cell cycle analysis in senescent cells. 33
Figure 4. miRNA expression levels in time. 38
Figure 5. Cell features changes after TGF-β1 treatment. 44