Title Page
ABSTRACT
Contents
Chapter 1. Introduction 9
Chapter 2. Itch and pain have distinct population in calcium imaging 20
Chapter 3. Itch and pain have distinct population in calcium imaging 29
Chapter 4. Itch and pain have their own network structure 35
Chapter 5. Behavior data shows itch has distinct response when mouse shows itch specific behavior 41
Chapter 6. Conclusion 45
References 49
국문초록 55
Figure 1. Data acquisition and processing steps (a) Experiment environment. Freely moving mice's behavior and calcium signal recorded... 23
Figure 2. Overlapping % of itch and pain ensembles (a) itch-pain overlap % (b) itch-itch overlap %. Overlapping % compared from the... 24
Figure 3. PSD(power spectral density) analysis implies itch and pain's controversial relationship... 26
Figure 4. Shannon entropy shows stimuli induces increase of Shannon entropy. FFF, HHH, FHF, HFH.... 27
Figure 5. Machine learning classification process and classification matrix (A) (left) overall procedure of classification process. (right) confusion matrix shows actual... 34
Figure 6. Physical distance and itch network property. (a) Raw representative calcium image after histamine injection. (b) Physical distance and... 39
Figure 7. Network property of itch and pain network, compared with scrambled data.... 39
Figure 8. Network metric comparison. Small worldness difference showed pain network is affected by drug stimuli.... 40
Figure 9. Time window shows pre-post △calcium signal efficiently. Different time window applied (1s~3s) to calculate mean intensity. Paired t-test. 44