Title Page
Abstract
Contents
List of Abbreviations 9
INTRODUCTION 10
MATERIALS AND METHODS 14
RESULTS 22
DISCUSSION 62
REFERENCES 70
국문초록 78
Figure 1. nArgBP2 KD in mature neurons does not result in morphological defects in existing dendritic spines. 35
Figure 2. Sustained expression of nArgBP2 during neuronal development, and confirmation of knock-down efficiency by... 36
Figure 3. nArgBP2 KD in mature neurons completely inhibits spine head enlargement during cLTP. 37
Figure 4. Expression levels of NMDA receptors, GluN1 and GluN2 were not altered with nArgBP2 KD in hippocampal neurons. 38
Figure 5. 3D geometry analysis of dendritic spines during cLTP. 39
Figure 6. Expression of nArgBP2-res in ArgBP2 KD neurons showed similar expression levels to endogenous nArgBP2 levels. 40
Figure 7. nArgBP2 forms condensates in the dendritic spines and these condensates are dispersed during chemical-LTP, which... 41
Figure 8. Conservation of putative CaMKII phosphorylation site sacross species, and the schematic figure of CaMKII phosphorylation-... 42
Figure 9. nArgBP2 forms condensates in the dendritic spines and these condensates are dispersed by CaMKIIα-mediated phosphorylation. 43
Figure 10. nArgBP2₉₅₉-₁₁₉₆ droplets in living hippocampal neurons. 44
Figure 11. nArgBP2 droplets are dissolved by 1,6-hexanediol in living hippocampal neurons. 45
Figure 12. Endogenous nArgBP2 also formed liquid-like condensates in spines that were dispersed by 1,6-hexanediol treatment. 46
Figure 13. Biomolecular condensate assembly of nArgBP2 in heterologous systems with multivalent intermolecular interactions... 47
Figure 14. Biomolecular condensate assembly of nArgBP2 in vitro and heterologous systems (a-d) 48
Figure 15. Biomolecular condensate assembly of nArgBP2 in heterologous and in vitro expression systems shows liquid-like... 49
Figure 16. Biomolecular condensate co-assembly of nArgBP2 with CaMKIIα and rapid dispersion by Ca²⁺/CaMKIIα-mediated phosphorylation. 51
Figure 17. WAVE1 binds to nArgBP2 and their interaction interferes with the phase-separating property of nArgBP2. 53
Figure 18. Spine enlargement caused by nArgBP2 during cLTP is mediated by WAVE1 interaction. 55
Figure 19. Model for the role of nArgBP2 in enlargement of dendritic spines during LTP. 57
Figure 20. nArgBP2₉₅₉-₁₁₉₆ also formed droplets in developing neurons but these droplets are reluctant to be dissolved by 1,6... 58
Figure 21. The NSE domain of nArgBP2 is required for the efficient targeting of nArgBP2 to dendritic spines. 59
Figure 22. The efficient targeting of nArgBP2 to the dendritic spine is zinc-dependent. 60
Figure 23. The schematic figure of role of nArgBP2. 61