Title Page
ABSTRACT
Contents
INTRODUCTION 10
MATERIALS AND METHODS 15
Patients 15
Ovum pick up and ICSI with spindle observation 15
Embryo Culture 16
Trophectoderm Biopsy 17
Blastocyst vitrification, warming, and embryo transfer 18
Statistical analysis 19
RESULTS 22
Discussion 36
REFERENCES 42
논문개요 48
Table 1. Reproductive characteristics of patients 23
Table 2. Mean of timepoints by spindle in the euploidy embryo groups 31
Table 3. Mean of timepoints by spindle in the aneuploidy embryo groups 32
Table 4. Mean of timepoints by spindle in the mosaicism embryo groups 33
Table 5. Percent of clinical outcomes based on spindle angle groups 35
Figure 1. Advanced of Preimplantation Genetic Testing technique 11
Figure 2. Process of study 20
Figure 3. Oocyte meiotic spindle view with polscope 21
Figure 4. Relationship between spindle localization, oocyte competence and embryo development 25
Figure 5. Comparison of euploid blastocyst rates in different spindle angles 27
Figure 6. Comparison of aneuploid blastocyst rates in different spindle angles 29