Title Page
ABSTRACT
초록
Contents
NOMENCLATURE 14
1. INTRODUCTION 16
2. MATERIAL AND METHOD 19
2.1. Materials and Reagents 19
2.2. Antioxidant Capacity and Component Assay 19
2.2.1. ABTS Radical Scavenging Assay 19
2.2.2. DPPH Radical Scavenging Assay 20
2.2.3. Total Polyphenol Content 20
2.2.4. Total Flavonoid Content 21
2.3. Cell Culture 21
2.4. Cell Viability 22
2.5. Cell Treatment for Protein Expression 23
2.6. Animals and Treatment 23
2.7. Histological Analysis 24
2.8. Immunoblot Analysis 24
2.9. Statistical Analysis 25
3. RESULTS 26
3.1. Antioxidant Capacity in CJB 26
3.2. Effect of CJB on cell viability in DEX-Treated C2C12 Myotubes 28
3.3. CJB Reduces Muscle Degradation in DEX-Treated C2C12 Myotubes 30
3.3.1. Effect of CJB on the Expression of Muscle Degradation-Regulating Factors in DEX-Treated C2C12 Myotubes 30
3.3.2. Effect of CJB on the Expression of Mitochondrial Biogenesis Factors in DEX-Treated C2C12 Myotubes 32
3.3.3. Effect of CJB on the Expression of NRF2 and HO-1 in DEX-Treated C2C12 Myotubes 34
3.4. The Administration of CJB May Reduce Atrophy Caused in DEX-Induced Muscle Atrophy Mice 36
3.4.1. Oral Administration of CJB Prevents DEX-Induced Calf Muscle Damage 36
3.4.2. Effects of the Administration of CJB on Serum Biochemistry 38
3.5. Effects of CJB on Protein Expression Levels in DEX-Induced Muscle Atrophy Mice 40
3.5.1. Effect of CJB on the Expression of Muscle Degradation-Regulating Factors in DEX-Induced Muscle Atrophy Mice 40
3.5.2. Effect of CJB on the Expression of Mitochondrial Biogenesis Factors in DEX-Induced Muscle Atrophy Mice 42
3.5.3. Effect of CJB on the Expression of NRF2 and HO-1 in DEX-Induced Muscle Atrophy Mice 44
4. DISCUSSION 46
5. CONCLUSION 52
REFERENCES 53
Table 1. Radical scavenging capacity in CJB 27
Table 2. Effect of CJB on ALT, AST, CK and LDH in DEX-administered mice 39
Figure 1. CJB increases cell viability on DEX-induced C2C12 myotubes 29
Figure 2. CJB decreased the protein expression of muscle degradation factors on DEX-induced C2C12 myotubes 31
Figure 3. CJB increased the protein expression of mitochondrial biogenesis factors on DEX-induced C2C12 myotubes 33
Figure 4. CJB increased the protein expression of antioxidant factors on DEX-induced C2C12 myotubes 35
Figure 5. Effects of CJB on the structural damage of muscle tissue in mice with DEX-treated muscle atrophy 37
Figure 6. CJB decreased the protein expression of muscle degradation factors in DEX-Induced Muscle Atrophy Mice 41
Figure 7. CJB increased the protein expression of mitochondrial biogenesis factors in DEX-Induced Muscle Atrophy Mice 43
Figure 8. CJB increased the protein expression of antioxidant factors in DEX-Induced Muscle Atrophy Mice 45