Title Page
Abstract
초록
PREFACE
Contents
CHAPTER 1. Introduction 11
CHAPTER 2. Theory 13
2.1. DNA sequence analysis 13
2.2. Ring cavity laser 16
CHAPTER 3. Experimental 17
3.1. DNA 17
3.2. Methods 19
CHAPTER 4. Results 23
4.1. DNA detection with OFRR laser 23
4.2. Analysis of individual DNA microdroplets 27
CHAPTER 5. Discussion 29
Reference 30
Table 3.1. DNA sequences of target and base-mismatched sample. 18
Fig. 2.1. Fluorescence in single-stranded and double-stranded of DNA combined with intercalating dyes. 14
Fig. 2.2. Melting curves according to DNA of wild type, mutant and heterozygote. 15
Fig. 3.1. Schematics of microfluidic system for DNA/dye microdroplet generation and optofluidic ring resonator (OFRR) laser experiments downstream. 21
Fig. 3.2. Schematics of optofluidic ring resonator (OFRR) laser 22
Fig. 4.1. Laser emission spectra from the target DNA and base-mismatched DNA when pumped with the same energy density 25
Fig. 4.2. Spectrally integrated laser emission intensity as a function of pump energy density. 26
Fig. 4.3. The optical signals observed by the spectrometer at the distal end of the fiber taper as a function of time. 28