Title Page
ABSTRACT
국문 초록
Contents
CHAPTER 1. Introduction 13
1.1. Research background 13
1.2. Purpose of Research 15
CHAPTER 2. Materials and Methods 17
2.1. Materials 17
2.2. Verification of DNA hydrogel formation 18
2.3. Synthesis of MWCNT/pHEMA 19
2.4. Polymer activation and primer immobilization 21
2.5. Validation of primer immobilization on the pHEMA 22
2.6. Preparation of templates to be immobilized to the primer on the MWCNT/pHEMA 23
2.7. Measurement of equilibrium water content (EWC) 24
2.8. Electrical measurement of a MWCNT/pHEMA sensor 25
2.9. Rolling circle amplification (RCA) reaction on MWCNT/pHEMA 25
CHAPTER 3. Results and Discussion 29
3.1. Basic characterization of CNT/pHEMA 29
3.2. Binding and immobilization of MWCNT/pHEMA 32
3.3. Stability of MWCNT/pHEMA 39
3.4. Electrical properties 44
CHAPTER 4. CONCLUSION 49
REFERENCES 50
Table 1. Nucleic acid sequences list 18
Figure 1. The structural diagram of pHEMA co-polymerization. 20
Figure 2. Schematic diagram of amine coupling reaction on MWCNT/pHEMA. 21
Figure 3. (A) A photograph of the DNA hydrogel formation over MWCNTs/pHEMA (B) no reaction with the caboxlyated pHEMA. 23
Figure 4. Experimental set-up and photographs for the electrical detection of MWCNT/pHEMA. A home-made acryl chamber contains two MWCNT/pHEMA... 27
Figure 5. Schematic diagram of DNA hydrogel formation by rolling circle amplification over the MWCNTs/pHEMA network.... 28
Figure 6. (A) The exemplary FT-IR spectrum of HEMA monomer, HEMA/ EGDMA, HEMA/EGDMA/AA and HEMA/EGDMA/MAA.... 31
Figure 7. Surface modification analysis of only HEMA and Amine modified pHEMA using X-ray spectroscopy.... 36
Figure 8. DNA hydrogel formation and rheological properties. (A) The captured picture shows liquid state when the non-target pathogen (MERS) is mis-... 37
Figure 9. Fluorescence microscopic images with fluorescence FAM probe with (A) carboxylic pHEMA and (B) amine modified pHEMA surface. 38
Figure 10. The equilibrium water content (EWC) of (A) five types of pHEMA composed of different comonomers. (B) comparison of the EWC in distilled (DI) water... 42
Figure 11. The different amount of the EGDMA as cross-linking agent (A) Optical image of polyHEMA hydrogel with different content of EGDMA (0.1-30... 43
Figure 12. Electrical properties of MWCNT/pHEMA. (A) Opical images of the pHEMA with initial, bending and twisting state (left),... 47
Figure 13. Electrical properties of DNA hydrogel. (A) I-V characteristic of pathogen matched DNA hydrogel (positive) and mismatched liquid state. (negative)... 48