Title Page
ABSTRACT
국문 초록
PREFACE
Contents
1. INTRODUCTION 14
1.1. Coherent Anti-Stokes Raman Scattering (CARS) imaging 14
1.2. CARS Applications in Imaging 15
1.2.3. Superiority of CARS imaging: Chemical analysis without staining 15
1.2.2. Challenge of CARS imaging in bio-sample: Shallow imaging depth 16
1.3. Previous Adaptive Optics (AO) Assisted CARS Microscopy Research and Their Limitations 16
1.4. Closed-Loop Accumulation of Single Scattering (CLASS) 17
1.4.1. Transmission Matrix and Reciprocity 17
1.4.2. Single Conjugate Closed-Loop Accumulation of Single Scattering (SCC) 23
1.5. Benefits of Combining CLASS and CARS Microscopy 26
2. METHOD 26
2.1. Hardware Setup and Sample Preparation 26
2.1.1. Reflection Matrix Measurement 27
2.1.2. Wavefront Modulation 28
2.1.3. CARS Detection 29
2.1.4. Sample Preparation 29
2.2. Software Processing1: Reflection Matrix Measurement 30
2.3. Software Processing2: Aberration Identification 32
2.4. Software Processing3: Correction Map Generation 33
2.5. Software Processing4: Averaging Correction Maps 35
3. RESULTS 37
3.1. Phantom Target 37
3.2. Ex-vivo MouseWhole Head 38
3.3. In-vivo MouseWhole Head 39
4. DISCUSSION 40
REFERENCES 42
SUPPLEMENTARY INFORMATION 44
A. AO-CARS Response Curve 44
B. CARS Intensity Response to Laser's Wavefront Shaping in Bulk Sample 45
SUPPLEMENTARY INFORMATION REFERENCES 47
Figure 1. Schematic diagram of CARS. 15
Figure 2. Concept of transmission matrix. 18
Figure 3. Dipole radiation. 19
Figure 4. Direction-oriented pictures of the scattering. 21
Figure 5. Basic correlation concept in SCC algorithm. 24
Figure 6. Reciprocity in SCC algorithm. 25
Figure 7. Setup. 27
Figure 8. Sample schemes. 29
Figure 9. Off-axis holography method. 31
Figure 10. Aberration Identification using the SCC algorithm. 33
Figure 11. Correction map generation from the skull aberrations. 34
Figure 12. Select averaging size of correction maps. 36
Figure 13. Phantom target, CARS improvement. 37
Figure 14. Ex-vivo mouse whole head, CARS improvement. 38
Figure 15. In-vivo mouse whole head, CARS improvement. 40
Figure S1. Averaging size of correction map and its effect on the CARS image. 45
Figure S2. CARS power behavior with different aberrations. 46