표제지
목차
국문요약 10
Ⅰ. 서론 12
Ⅱ. 실험 재료 및 방법 14
1. 시약 14
2. 실험방법 15
2.1. Paspalum thunbergii 추출물 제조 15
Ⅲ. 결과 22
1. P. thunbergii 추출물의 항산화 효과 22
1.1. DPPH법와 ABTS법을 이용한 라디칼 소거 활성 분석 22
2. P. thunbergii 추출물의 세포 생존율 분석 24
3. P. thunbergii 추출물의 항염증 효과 26
3.1. P. thunbergii 추출물의 NO 생성 저해 효과 26
3.2. Western blot을 통한 항염증 관련 시그널 단백질 발현 변화 28
3.3. Confocal microscope를 통한 NF-kB의 핵 내 이동 억제 확인 34
4. P. thunbergii 추출물의 멜라닌 합성 저해 효과 36
5. P. thunbergii 추출물의 콜라겐 합성 촉진 효과 38
5.1. P. thunbergii 추출물에 의한 MMPs 활성 억제효과 38
5.2. Western blot을 통한 MMPs 관련 시그널 단백질 발현 변화 40
5.3. P. thunbergii 추출물에 의한 콜라겐 합성 증가 효과 42
Ⅳ. 결론 44
Ⅴ. 참고문헌 48
Abstract 57
Figure 1. Anti-oxidative activity of P. thunbergii extract. 23
Figure 2. Effects of P. thunbergii extract on the cell viability. 25
Figure 3. Effect of P. thunbergii extract on NO production of Raw 264.7 cells. 27
Figure 4. Effects of P. thunbergii extract on the expression of COX-2, iNOS in LPS-induced Raw 264.7 cells via western blot. 29
Figure 5. Effects of P. thunbergii extract on the expression of p38, JNK, ERK in LPS-induced Raw 264.7 cells via western blot. 30
Figure 6. Effects of P. thunbergii extract on the expression of IKKβ, IkBα, NF-kB in LPS-induced Raw 264.7 cells via western blot. 31
Figure 7. Effects of P. thunbergii extract on the expression of JAK1, STAT3 in LPS-induced Raw 264.7 cells via western blot. 32
Figure 8. Effects of P. thunbergii extract on the expression of Wnt 3α, β-catenin, GSK-3β in LPS-induced Raw 264.7 cells via western blot. 33
Figure 9. P. thunbergii extract blocks LPS-induced translocation of p-NF-kB into the nucleus. 35
Figure 10. Inhibition of melanogenesis by P. thunbergii extract. 37
Figure 11. Effects of P. thunbergii extract on the activity of MMP-2 and MMP-9 via gelatin zymography. 39
Figure 12. Effects of P. thunbergii extract on the expression of MMP-2 and MMP-9 in UVB-induced HaCaT cells via western blot. 41
Figure 13. Determination of soluble collagen biosynthesis in the presence of P. thunbergii extract via sircol collagen assay. 43