표제지
목차
Abstract 18
Ⅰ. 서설 21
Ⅱ. 한국 남부 해역에 출현하는 깨다시꽃게 유생의 시·공간적 분포 27
1. 서론 27
2. 재료 및 방법 31
2.1. 환경자료 수집 31
2.2. 시료 채집 31
2.3. 유생의 동정 34
2.4. 유생의 발달단계별 그룹화 36
2.5. 자료 분석 38
3. 결과 39
3.1. 환경자료 분석 39
3.2. 유생의 출현 및 분포 48
3.3. 환경자료와 유생밀도 사이의 상관관계 56
4. 고찰 62
Ⅲ. 한국 서해에 서식하는 깨다시꽃게의 산란 생태학적 연구 69
1. 서론 69
2. 재료 및 방법 72
2.1. 시료 채집 72
2.2. 환경자료 수집 74
2.3. 계측형질 측정 75
2.4. 성별구분 및 생식소 성숙단계의 육안판별 77
2.5. 비만도와 생식소숙도지수의 분석 78
2.6. 조직학적 분석 79
2.7. 난모세포 크기 빈도의 월변화 분석 81
2.8. 군성숙갑폭 분석 81
2.9. 자료 분석 82
3. 결과 83
3.1. 환경자료 분석 83
3.2. 성숙과 산란 86
4. 고찰 119
Ⅳ. 한국 서해에 서식하는 깨다시꽃게의 성장과 개체군 특성 125
1. 서론 125
2. 재료 및 방법 127
2.1. 상대성장 분석 127
2.2. 성장식 분석 130
2.3. 개체군 특성 분석 132
2.4. 자료 분석 133
3. 결과 134
3.1. 상대성장 134
3.2. 성장 유형 분류 142
3.3. 성적이형 144
3.4. 개체군 특성 147
4. 고찰 156
Ⅴ. 한국 연근해에 출현하는 깨다시꽃게 개체군의 유전학적 분석 164
1. 서론 164
2. 재료 및 방법 166
2.1. 시료 채집 166
2.2. Genomic DNA 추출 및 분석 168
2.3. 자료 분석 171
3. 결과 172
3.1. mtDNA 분석 172
3.2. 해역별 유전적 분화도와 다양성 178
3.3. 집단 유전학적 분석 181
4. 고찰 187
Ⅵ. 종합 결론 190
Ⅶ. 참고 문헌 195
Table 1. Grouping each larval stage of Ovalipes punctatus 37
Table 2. Range of sea surface temperature (SST) and salinity (SSS) in 2019 42
Table 3. Range of sea surface chlorophyll α (SSC) in 2019 46
Table 4. Monthly occurrence of each Ovalipes punctatus larval stage 51
Table 5. Summary of redundancy analysis between densities of each larval stage and environmental factors 60
Table 6. Spearman's rank correlation between densities of each larval stage and environmental factors 61
Table 7. Range of sea surface temperature (SST) and salinity (SSS) in the Yellow Sea of Korea in 2021 84
Table 8. Monthly sex ratio of Ovalipes punctatus in the Yellow Sea of Korea in 2021 87
Table 9. Monthly carapace width distribution of Ovalipes punctatus in the Yellow Sea of Korea in 2021 91
Table 10. Monthly chela length of Ovalipes punctatus in the Yellow Sea of Korea in 2021 92
Table 11. Monthly abdominal width of Ovalipes punctatus in the Yellow Sea of Korea in 2021 93
Table 12. Monthly orbital spine width of Ovalipes punctatus in the Yellow Sea of Korea in 2021 94
Table 13. Monthly body weight of Ovalipes punctatus in the Yellow Sea of Korea in 2021 95
Table 14. Characteristic of ovarian stages in Ovalipes punctatus 97
Table 15. Monthly condition factor (K) of Ovalipes punctatus in the Yellow Sea of Korea in 2021 100
Table 16. Monthly gonadosomatic index of Ovalipes punctatus in the Yellow Sea of Korea in 2021 103
Table 17. Range of abdominal width (AW) and gonadosomatic index (GSI) at each gonadal stage of the Ovalipes punctatus in the Yellow Sea of Korea... 108
Table 18. Allometric growth equations and growth type of Ovalipes punctatus in the Yellow Sea of Korea in 2021 143
Table 19. Montly modes separation of length frequency distribution of Ovalipes punctatus using by Bhattacharya's method 149
Table 20. Modified von Bertalanffy growth parameters estimation by the ELEFAN analysis of length frequency data for males and females 151
Table 21. Monthly recruitment rate of Ovalipes punctatus as estimated by ELEFAN II routine 154
Table 22. The number of samples in each sampling area 170
Table 23. Primer sequence for the PCR amplification 170
Table 24. Sequence lists from Genebank 173
Table 25. Distribution of mtDNA haplotype of Ovalipes puctatus for each sampling area 174
Table 26. Pairwise FST values (below diagonal) and Pairwise FST p values (above diagonal) for the mtDNA COI by each sampling area of Ovalipes punctatus[이미지참조] 179
Table 27. Haplotype and nucleotide diversity based on the mtDNA COI by each sampling area of Ovalipes punctatus 180
Table 28. Molecular diversity of Ovalipes punctatus for each sampling area 182
Fig. 1. Total yield of commercial crabs from 2010 to 2022 in Korea. 26
Fig. 2. Photograph of Ovalipes punctatus. 26
Fig. 3. Total sampling stations of Korean waters in 2019. 33
Fig. 4. Larval stages of Ovalipes punctatus. A, Zoea; B, Megalopa; C, Juvenile. Red arrow indicate maxilla of Zoea [Setation of endopod (4+3) were represented by the black dashed line box in Fig. 2A]. 35
Fig. 5. Horizontal distributions of sea surface temperature (SST) in 2019. A, February; B, March; C, April; D, May; E, June; F, August; G, September; H, October; I, December. 40
Fig. 6. Horizontal distributions of sea surface salinity (SSS) in 2019. A, February; B, March; C, April; D, May; E, June; F, August; G, September; H, October; I, December. 41
Fig. 7. Monthly variation of sea surface temperature (A) and salinity (B) in 2019. Vertical bars indicate S.D.. 43
Fig. 8. Horizontal distributions of sea surface chlorophyll α (SSC) in 2019. A, April; B, May; C, August; D, September. 45
Fig. 9. Monthly variation of sea surface chlorophyll α in 2019. Vertical bars indicate S.D.. 47
Fig. 10. Monthly variation of total density of Ovalipes punctatus larvae in Korean waters in 2019. 49
Fig. 11. Density variation of larval stage groups by periods in Korean waters in 2019. 4L, Later April; 5E, Early May; 5L, Later May; 6E, Early June. 53
Fig. 12. Distribution patterns of each larval stage of Ovalipes punctatus in 2019. A, April; B, May; C, June. Red asterisks (*) indicate no larval occurrence. 55
Fig. 13. The temperature, salinity and density diagram of Ovalipes punctatus larvae in Korean waters in 2019. SST, sea surface temperature; SSS, sea... 57
Fig. 14. Redundancy analysis biplot for densities of each larval stage with sea surface temperature (SST), sea surface salinity (SSS) and sea surface... 59
Fig. 15. Sampling area of Ovalipes punctatus in Korean waters in 2021. Coordinate of sampling area: 35° 15' 00" N, 125° 15' 00" E. 73
Fig. 16. Morphometric measurements of Ovalipes punctatus. A, Orbital spine width (OW); B, Carapace width (CW); C, Abdominal width (AW); D, Chela... 76
Fig. 17. Gonad of female Ovalipes punctatus. OL, ovarian lobe; TC, transverse commissure. 80
Fig. 18. Monthly variation of sea surface temperature (A) and salinity (B) in the Yellow Sea of Korea in 2021. Vertical bars indicate S.D.. 85
Fig. 19. Monthly variation of Ovalipes punctatus sex ratio in the Yellow Sea of Korea in 2021. 88
Fig. 20. Length frequency distribution of Ovalipes punctatus in the Yellow Sea of Korea in 2021. 90
Fig. 21. Photograph of gonad by different developmental stages in Ovalipes punctatus. A, immature; B, maturing; C, mature; D, ripe. 98
Fig. 22. Monthly variation of condition factor (K) of Ovalipes punctatus in the Yellow Sea of Korea in 2021. Vertical bars indicate S.E.. 101
Fig. 23. Monthly variation of gonadosomatic index of Ovalipes punctatus in the Yellow Sea of Korea in 2021. Vertical bars indicate S.E. 104
Fig. 24. Monthly changes in maturity stages of Ovalipes punctatus in the Yellow Sea of Korea in 2021. 106
Fig. 25. Relationship between abdominal width and gonadosomatic index at each gonadal stage of the Ovalipes punctatus in the Yellow Sea of Korea in 2021. 109
Fig. 26. Photograph of abdominal flap by different maturation stages in Ovalipes punctatus. 110
Fig. 27. Photomicrographs of each ovarian development stages in Ovalipes punctatus with hematoxylin and eosin staining. A, immature (early); B,... 113
Fig. 28. Frequency distribution of oocyte diameter of the Ovalipes punctatus in the Yellow Sea of Korea from April to September in 2021. 116
Fig. 29. Relationship between carapace width (CW) and group maturity of female Ovalipes punctatus in the Yellow Sea of Korea in 2021. L₅₀%M is...[이미지참조] 118
Fig. 30. Relationship between carapace width and body weight of Ovalipes punctatus in the Yellow Sea of Korea in 2021. 135
Fig. 31. Relationship between carapace width and chela length of Ovalipes punctatus in the Yellow Sea of Korea in 2021. 137
Fig. 32. Relationship between carapace width and orbital spine width of Ovalipes punctatus in the Yellow Sea of Korea in 2021. 139
Fig. 33. Relationship between carapace width and abdominal width of Ovalipes punctatus in the Yellow Sea of Korea in 2021. 141
Fig. 34. Body weight frequency distribution of Ovalipes punctatus in the Yellow Sea of Korea in 2021. 145
Fig. 35. Chela length frequency distribution of Ovalipes punctatus in the Yellow Sea of Korea in 2021. 145
Fig. 36. Orbital spine width frequency distribution of Ovalipes punctatus in the Yellow Sea of Korea in 2021 146
Fig. 37. Abdominal width frequency distribution of Ovalipes punctatus in the Yellow Sea of Korea in 2021. 146
Fig. 38. Montly modes separation of length frequency distribution and normal distribution curves of Ovalipes punctatus using by NORMSEP. 148
Fig. 39. The modified von Bertalanffy growth curves for male and female of Ovalipes punctatus based on length frequency distributions. CW∞ were...[이미지참조] 152
Fig. 40. The annual recruitment pattern of Ovalipes punctatus as estimated by ELEFAN II routine. 155
Fig. 41. Sampling area of Ovalipes punctatus in Korean waters in 2021. ES, East Sea; YS, Yellow Sea; SS, South Sea; ID, Ieodo. 167
Fig. 42. The phylogenetic tree based on the Neighbor-joining (NJ) method of Ovalipes punctatus with China sequences from Genebank. Bootstrap values... 177
Fig. 43. Mismatch distribution of Ovalipes punctatus for each sampling area from sudden expansion model. ES, East Sea; YS, Yellow Sea; SS, South Sea;... 183
Fig. 44. Haplotype distribution by clade of Ovalipes punctatus for each sampling area. 185
Fig. 45. A single minimum spanning tree of the 78 mtDNA COI haplotypes of Ovalipes punctatus. Circle size reflects haplotype abundances. The length... 186