Title Page
Contents
요약 8
1. Introduction 9
2. Materals & Methods 11
2.1. Surgical preparation of animals 11
2.2. Western blotting 12
2.3. Realtime PCR 13
2.4. Sirius red staining & Masson's trichrome staining 14
2.5. Immunohistochemical(IHC) staining 15
2.6. Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assays 16
2.7. Histology (PAS staining) 17
2.8. ELISA for Angiotensin (1-7), Angiotensin II 18
2.9. Statistical analysis 19
3. Results 20
3.1. DIZE exacerbates UUO-induced renal interstitial fibrosis 20
3.2. Diminazene aceturate aggravates unilateral ureteral obstruction-induced histological tubular damage and apoptotic cell death in kidneys 28
3.3. Diminazene aceturate treatment has no effect on angiotensin-converting enzyme 2 activity or angiotensin (1-7) and angiotensin II levels after unilateral ureteral obstruction 31
3.4. Diminazene aceturate aggravates inflammation and infiltration of macrophages in ureteral-obstructed kidneys 33
3.5. Diminazene aceturate aggravates profibrotic M2 macrophage accumulation after unilateral ureteral obstruction 41
4. Discussion 44
Reference 49
Fig. 1. DIZE exacerbates UUO-induced fibrosis in the kidneys of female mice. Mice were subjected to right unilateral ureteral obstruction. Male... 22
Fig. 2. DIZE exacerbates UUO-induced collagen deposition in the kidneys of female mice. Mice were subjected to right unilateral ureteral... 26
Fig. 3. DIZE aggravates UUO-induced histological tubular damage and apoptotic cell death in female mouse kidneys. Mice were subjected to... 29
Fig. 4. DIZE treatment does not affect ACE2 mRNA expression or protein levels of angiotensin II and angiotensin (1-7) in UUO female... 32
Fig. 5. DIZE aggravates infiltration of macrophages in the ureteral obstructed kidneys of female mice. Female mice were subjected to right... 35
Fig. 6. DIZE aggravates inflammation in ureteral obstructed kidneys of female mice. Female mice were subjected to right unilateral ureteral... 37
Fig. 7. DIZE induces M2 macrophage polarization. Female mice were subjected to right unilateral ureteral obstruction. Mice were treated with... 42