Title Page
Contents
요약 8
1. Introduction 9
2. Materials & Methods 12
2.1. Animal preparation 12
2.2. Measurement of kidney functional and histological damages 14
2.3. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays and immunohistochemistry (IHC) staining 15
2.4. Western blotting 16
2.5. Quantitative RT-PCR 17
2.6. Statistical analysis 18
3. Results 19
3.1. Oxypurinol pretreatment protects the kidney against I/R injury via HO-1 induction 19
3.2. Oxypurinol pretreatment protects against apoptotic tubular cell death after renal I/R injury 24
3.3. Oxypurinol pretreatment attenuates kidney neutrophil infiltration after renal I/R injury 27
3.4. Oxypurinol pretreatment attenuates oxidative damage after renal I/R injury 31
4. Discussion 35
Reference 39
Fig. 1. Oxypurinol protects against renal ischemia/reperfusion injury via heme oxygenase-1 (HO-1) induction. 21
Fig. 2. Oxypurinol protects renal tubular apoptotic cell death after renal ischemia/reperfusion injury. 25
Fig. 3. Oxypurinol reduces neutrophil infiltration and Ly6G protein expression after renal ischemia/reperfusion injury. 28
Fig. 4. Oxypurinol reduces oxidative damage after renal ischemia/reperfusion injury. 32