Title Page
Contents
ABBREVIATIONS 9
국문초록 10
Ⅰ. Water extract of Glycyrrhiza uralensis Fisch. ex DC. inhibits the prostatic cell growth by promoting cell cycle arrest and apoptosis in vitro and in vivo 12
ABSTRACT 13
INTRODUCTION 15
MATERIALS AND METHODS 18
RESULTS 26
DISCUSSION 43
CONCLUSION 46
REFERENCES 47
Ⅱ. Umbelliferone ameliorates benign prostatic hyperplasia by inhibiting cell proliferation and G1/S phase cell cycle progression through regulation of STAT3/E2F1 axis 51
ABSTRACT 52
INTRODUCTION 54
MATERIALS AND METHODS 57
RESULTS 65
DISCUSSION 81
CONCLUSION 85
REFERENCES 86
PART Ⅰ 25
Table 1. Information on oligonucleotide primers for rat 25
PART Ⅱ 61
Table 1. Information on oligonucleotide primers for human 61
PART Ⅰ 27
Figure 1. Effect of EGU treatment on cell proliferation mediated by AR signaling pathway in BPH-1 cells. 27
Figure 2. Effect of EGU treatment on cell cycle progression at G1/S phase in BPH-1 cells. 29
Figure 3. Effect of EGU treatment on caspase-dependent apoptosis in BPH-1 cells. 32
Figure 4. Effect of EGU administration on the enlargement of prostate in TP-induced BPH rats. 35
Figure 5. Effect of EGU administration on androgen-mediated AR signaling pathway in TP-induced BPH rats. 38
Figure 6. Effect of EGU administration on cell cycle progression at G1/S phase in TP-induced BPH rats. 40
Figure 7. Effect of EGU administration on caspase-dependent apoptosis in TP-induced BPH rats. 42
PART Ⅱ 67
Figure 1. Effect of UMB on the blockade of androgen/AR signaling in BPH-1 cells. 67
Figure 2. Effect of UMB on cell cycle arrest at the G1/S phase in BPH-1 cells. 70
Figure 3. Effect of UMB treatment on E2F1 expression in BPH-1 cells. 72
Figure 4. Effect of UMB treatment on TGFβ1 and p-STAT3 expression in BPH-1 cells. 74
Figure 5. Effect of UMB on prostate enlargement in TP-induced rats. 77
Figure 6. Effect of UMB on histological alterations in TP-induced rats. 80