Title Page
Contents
ABSTRACT 7
INTRODUCTION 9
MATERIALS AND METHODS 12
1. Sample preparation 12
2. Experimental animals 13
3. Histological analysis 14
4. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis 14
5. Western blotting 15
6. Immunofluorescence assay 16
7. Statistical analysis 17
RESULTS 18
1. Effect of SC-H on the body weight gain in HFD-induced obese mice 18
2. Effect of SC-H on the weight and lipid accumulation in the scWAT of HFD-induced obese mice. 20
3. Effect of SC-H on the expression of p-AMPK and PPAR γ in the scWAT of HFD-induced obese mice. 22
4. Effect of SC-H on the adipocyte browning specific markers in the scWAT of HFD-induced obese mice. 24
5. Effect of SC-H on the immunofluorescence intensity of UCP1 in the scWAT of HFD-induced obese mice. 26
6. Effect of SC-H on the weight and lipid accumulation in the iBAT of HFD-induced obese mice. 28
7. Effect of SC-H on the expression of p-AMPK in the iBAT of HFD-induced obese mice. 30
8. Effect of SC-H on the adipocyte browning specific markers in the iBAT of HFD-induced obese mice. 32
9. Effect of SC-H on the immunofluorescence intensity of UCP1 in the iBAT of HFD-induced obese mice. 34
DISCUSSION 36
CONCLUSION 41
REFERENCES 43
국문초록 49
Figure 1. Effect of SC-H on the body weight gain in HFD-induced obese mice. 19
Figure 2. Effect of SC-H on the weight and lipid accumulation in the scWAT of HFD-induced obese mice. 21
Figure 3. Effect of SC-H on the p-AMPK and PPARγ expression in the scWAT of HFD-induced obese mice. 23
Figure 4. Effect of SC-H on the adipocyte browning specific markers in the scWAT of HFD induced obese mice. 25
Figure 5. Effect of SC-H on the immunofluorescence intensity of UCP1 in the scWAT of HFD-induced obese mice. 27
Figure 6. Effect of SC-H on the weight and lipid accumulation in the iBAT of HFD-induced obese mice. 29
Figure 7. Effect of SC-H on the expression of p-AMPK in the iBAT of HFD-induced obese mice. 31
Figure 8. Effect of SC-H on the adipocyte browning specific markers in the iBAT of HFD-induced obese mice. 33
Figure 9. Effect of HME-SCs on the immunofluorescence intensity of UCP1 in the iBAT of HFD-induced obese mice. 35
Figure 10. Schematic diagram of mechanism of SC-H2 on the anti-obesity effect through adipose tissue browning 42