표제지
목차
요약 9
Ⅰ. 서론 10
Ⅱ. 재료 및 실험방법 13
1. 실험재료 13
2. 세포배양 13
3. 세포독성 평가 13
4. Real-time RT-PCR 13
5. Enzyme-linkes immunosorbent assay (ELISA) 14
6. Western blot 14
7. 통계처리 15
Ⅲ. 실험 결과 18
1. Hs68 세포에서 esculetin의 세포독성평가 18
2. Hs68 세포에서 esculetin의 TNF-α로 유도된 MMP-1 mRNA 발현 및 단백질 분비 억제 효과 20
3. Hs68 세포에서 esculetin에 의한 MAPKs 인산화 22
4. Hs68 세포에서 esculetin에 의한 mTOR/p70S6K 인산화 저해 25
5. Hs68세포에서 esculetin에 의한 NF-κB 인산화 저해 28
Ⅳ. 결론 31
Ⅴ. 참고문헌 32
Abstract 37
Table 1. Gene symbol, name and assay ID number in real-time PCR 16
Table 2. Antibody name and assay ID number in western blot analysis 17
Figure 1. Chemical structure and cell viability of esculetin on Hs68 cells. (A) The chemical structure of esculetin. (B) Cell viability of esculetin.... 19
Figure 2. Effect of esculetin on MMP-1 mRNA expression and secretion in TNF-α induced Hs68 cells. Forskolin is positive control. (A) Esculetin... 21
Figure 3. Effect of esculetin on MAPKs signaling pathway protein expression in Hs68 cells. (A) Esculetin did not inhibit MAPKs... 24
Figure 4. Effect of Esculetin on mTOR-p70S6K signaling pathway protein expression in Hs68 cells. (A) Esculetin inhibited mTOR-p70S6K... 27
Figure 5. Effect of Esculetin on NF-κB signaling pathway protein expression in Hs68 cells. (A) Esculetin inhibited NF-κB phosphorylation.... 29
Figure 6. Esculetin inhibited the expression of MMP-1 through inhibition of phosphorylation of mTOR/p70S6K and NF-κB. 30