표제지
목차
요약 7
Ⅰ. 서론 9
Ⅱ. 재료 및 실험방법 11
1. 실험재료 11
2. 세포배양 11
3. 세포 사멸능 평가 11
4. Quantitative real-time PCR (qPCR)을 통한 mRNA 발현 측정 12
5. Western blotting을 이용한 단백질 발현 측정 12
6. 통계 분석 13
Ⅲ. 결과 및 고찰 17
1. Esculetin의 피부암 세포 사멸능 측정 17
2. Esculetin의 G1/S기 조절 인자(cyclin D1) mRNA 발현 조절 19
3. Esculetin 처리 후 시간에 따른 세포 증식 및 세포 사멸 단백질 발현 22
4. Esculetin 농도에 따른 세포 증식 및 세포 사멸 단백질 발현 24
5. Esculetin의 MAPKs pathway 조절 28
Ⅳ. 결론 36
Ⅴ. 참고문헌 37
Abstract 40
Table 1. Gene name and assay ID number in real-time qPCR analysis 14
Table 2. Primer sequences in real-time qPCR analysis 15
Table 3. Antibody name and assay ID number in western blotting analysis 16
Figure 1. Esculetin suppresses skin cancer cell viability and growth. Cell viability was assessed for 24, 48, and 72 h in the (A) 0% FBS or (B)... 18
Figure 2. Esculetin suppresses cyclin D1 at the mRNA level in a time and concentration dependent manner. (A) Esculetin 0.8 mM was treated... 21
Figure 3. Esculetin suppresses skin cancer cell cycle progression and induces apoptosis in a time dependent manner. (A) Esculetin 0.8 mM... 23
Figure 4. Esculetin suppresses skin cancer cell cycle progression and induces apoptosis in a concentration dependent manner. (A) Esculetin... 27
Figure 5. Esculetin consequently suppresses skin cancer through the MAPKs pathway. (A) Esculetin (0.2, 0.4 and 0.8 mM) was treated with... 32
Figure 6. Esculetin can induce inhibition of A431 cell growth and proliferation by inhibiting cyclin D1. 33
Figure 7. Esculetin can induce A431 cell apoptosis by activating caspase-3 pathway. 34
Figure 8. Esculetin can suppresses the growth and proliferation of skin cancer by inhibiting the MAPKs pathway. 35