표제지
논문개요
목차
I. 서론 13
II. 재료 및 방법 23
1. 실험 재료 23
1) 시료 및 세포 23
2) 시약 및 기기 23
2. 실험 방법 26
1) 십자화과 채소류 추출물 제조 26
2) 케일 추출물 및 분획물 제조 26
3) HepG2 세포 배양 27
4) 세포 독성 측정 (Cell cytotoxicity) 29
5) 세포 증식 측정 (Cell proliferation) 30
6) 항산화능 측정 31
7) 항산화 성분 함량 측정 33
8) Proteasome 역가 저해율 측정 34
9) Western blot 36
10) LC-MS를 이용한 proteasome 억제 물질 분석 38
11) 통계처리 43
III. 결과 및 고찰 44
1. 십자화과 채소의 세포 독성 (Cell cytotoxicity) 44
2. 십자화과 채소의 세포 증식 (Cell proliferation) 44
3. 십자화과 채소의 항산화능 49
1) DPPH 라디칼 소거능 49
2) ABTS 라디칼 소거능 49
3) 세포 내 항산화능 (CAA) 49
4. 십자화과 채소의 항산화 성분 함량 53
1) 총 폴리페놀 함량 53
2) 총 플라보노이드 함량 53
5. 십자화과 채소의 proteasome 역가 저해율 56
1) 십자화과 채소의 proteasome 역가 저해율 스크리닝 56
2) 케일의 농도별 proteasome 역가 저해율 59
6. Genistein 및 배당체의 proteasome 역가 저해율 61
7. 케일에 의한 NF-κB 활성 변화 63
8. Proteasome 억제 물질 분석 65
1) 케일 추출물 및 분획물의 proteasome 역가 저해율 비교 65
2) 케일 추출물 및 분획물의 LC-MS 분석 67
3) 적양배추 추출물의 LC-MS 분석 73
IV. 결론 75
참고문헌 79
ABSTRACT 93
Table 1. LC-MS analysis conditions of kale extract and fractions. 39
Table 2. LC-MS analysis conditions of lutein in kale. 40
Table 3. LC-MS analysis conditions of phenolic compounds in kale. 41
Table 4. LC-MS analysis conditions of anthocyanins in red cabbage extract. 42
Table 5. Retention time and MS data of anthocyanins in red cabbage extract. 74
Fig. 1. Composition of 26S proteasome. 14
Fig. 2. Overview of the ubiquitin proteasome system. 16
Fig. 3. The schematic diagram showing the regulation NF-κB functions by the 26S proteasome. 18
Fig. 4. Extraction and fraction procedure of kale. 28
Fig. 5. Effects of cabbage extracts (A), red cabbage extracts (B), kohlrabi extracts (C), broccoli extracts (D), kale extracts (E) on... 48
Fig. 6. DPPH (A), ABTS (B) scavenging activity and cellular antioxidant activity value (C) of cabbage, red cabbage, kohlrabi,... 52
Fig. 7. Total phenolics (A) and flavonoid (B) contents of cabbage, red cabbage, kohlrabi, broccoli, kale extracts. 55
Fig. 8. Proteasome inhibitory activities of cabbage, red cabbage, kohlrabi, broccoli, kale extracts. (A): cell extract proteasome... 58
Fig. 9. Cellular proteasome inhibitory activity of kale extract. 60
Fig. 10. Proteasome inhibitory activities of Genistein and its glycosides. (A): cell extract proteasome inhibitory activity (CE... 62
Fig. 11. Effect of kale extract on TNF-α-induced NF-κB activation. 64
Fig. 12. Proteasome inhibitory activities of kale extract and solvent fractions. 66
Fig. 13. Chromatograms of kale extract and fractions at detector wavelength 320nm. 69
Fig. 14. Chromatograms of kale extract and fractions at detector wavelength 450nm. 70
Fig. 15. Chromatograms of lutein standard and kale hexane fraction. 71
Fig. 16. Chromatograms of kale ethyl acetate fraction. 72
Fig. 17. Chromatograms of anthocyanins in red cabbage extract. 74