표제지
목차
초록 15
제1장 서론 18
제2장 본론 22
제1절 천일염 및 생산단계의 미생물학적 위생평가 및 안전성 22
1. 서언 22
2. 실험 재료 및 방법 24
1) 실험 재료 24
2) 천일염 생산 단계별 미생물 분포도 24
3) 미생물 동정 26
4) 천일염 및 생산단계에서 접종된 병원성미생물의 생존 가능성 분석 26
5) 통계처리 26
3. 결과 및 고찰 27
1) 천일염 생산 단계별 미생물 분포도 27
2) 16S rRNA 염기서열 분석을 통한 분리균 동정 32
3) 천일염 및 생산단계에서 병원성미생물의 생존 가능성 분석 50
4. 요약 80
제2절 천일염 및 생산단계의 미세플라스틱 분석 및 저감화 82
1. 서언 82
2. 실험 재료 및 방법 84
1) 실험 재료 84
2) 미세플라스틱 분석 시료 전처리 84
3) 미세플라스틱 제조 및 크기 분류 84
4) Nile red staining 86
5) 미세플라스틱 첨가 염수 제조 86
6) 미세플라스틱 저감화 필터 86
7) 천일염 제조 및 전처리 88
8) Particle counter analysis 90
9) UV-Vis spectroscopy 및 형광현미경 관찰 90
10) Raman spectroscopy 분석 90
3. 결과 및 고찰 91
1) 천일염 생산공정에서 미세플라스틱 확인 91
2) 필터 장치를 이용한 염수의 미세플라스틱 저감화 96
3) 필터 장치를 이용하여 생산된 천일염 분석 102
4. 요약 109
제3절 함수정화장치를 활용한 천일염 생산 공정 개선 111
1. 서언 111
2. 실험 재료 및 방법 113
1) 함수정화장치 필터 113
2) Particle counter analysis 113
3) 실험용 간이 염전 및 천일염 생산 113
4) 천일염 생산량 및 입자 크기 분포 113
5) 천일염 성분 분석 115
6) 색도 분석 116
7) 통계처리 116
3. 결과 및 고찰 117
1) 함수정화장치 및 필터 개발 117
2) 함수정화장치 필터의 염수 정화 능력 122
3) 함수정화장치를 이용하여 생산된 천일염 분석 128
4. 요약 140
참고문헌 142
ABSTRACT 155
Table 1. Total bacterial counts isolated from solar salt during the entire manufacturing process(Shinan-gun saltern Ⅰ) 28
Table 2. Total bacterial counts isolated from solar salt during the entire manufacturing process(Shinan-gun saltern Ⅱ) 29
Table 3. Total bacterial counts isolated from solar salt during the entire manufacturing process(Shinan-gun saltern Ⅲ) 30
Table 4. Total bacterial counts isolated from solar salt during the entire manufacturing process (Yeonggwang-gun saltern Ⅰ) 31
Table 5. Species-identification of total aerobic bacteria isolated from saltern by 16S rRNA sequencing 34
Table 6. Species-identification of marine bacteria isolated from saltern by 16S rRNA sequencing 41
Table 7. Species-identification of moderate halophilic bacteria isolated from saltern by 16S rRNA sequencing 48
Table 8. Polymer number by size after filtration of the microplastic added in brine water by particle size analyzer 97
Table 9. Total suspended solids after filtration in brine water 123
Table 10. Micro particle number by size after filtration of brine water by particle size analyzer 125
Table 11. Hunter's color value by after filtration in brine water 127
Table 12. Production amount and yield of solar salt produced using filter equipment 129
Table 13. Particle size ratio of solar salt produced using filter equipment 131
Table 14. Hunter's color value of solar salt produced using filter equipment 134
Table 15. Contents of NaCl and Cl in solar salt produced using filter equipment 136
Table 16. Contents of inosoluble matters and sand in solar salt produced using filter equipment 137
Table 17. Contents of macro positive ion in solar salt produced using filter equipment 139
Figure 1. General structure of the saltern. 25
Figure 2. Phylogenetic position of total aerobic bacteria isolated from reservoir on 16S rRNA gene sequence. 35
Figure 3. Phylogenetic position of total aerobic bacteria isolated from evaporation pond(1st) on 16S rRNA gene sequence. 36
Figure 4. Phylogenetic position of total aerobic bacteria isolated from evaporation pond(2nd) on 16S rRNA gene sequence. 37
Figure 5. Phylogenetic position of total aerobic bacteria isolated from evaporation pond(3rd) on 16S rRNA gene sequence. 38
Figure 6. Phylogenetic position of total aerobic bacteria isolated from crystallization pond on 16S rRNA gene sequence. 39
Figure 7. Phylogenetic position of marine bacteria isolated from reservoir on 16S rRNA gene sequence. 42
Figure 8. Phylogenetic position of marine bacteria isolated from evaporation pond(1st) on 16S rRNA gene sequence. 43
Figure 9. Phylogenetic position of marine bacteria isolated from evaporation pond(2nd) on 16S rRNA gene sequence. 44
Figure 10. Phylogenetic position of marine bacteria isolated from evaporation pond(3rd) on 16S rRNA gene sequence. 45
Figure 11. Phylogenetic position of marine bacteria isolated from crystallization pond on 16S rRNA gene sequence. 46
Figure 12. Phylogenetic position of moderate halophilic bacteria isolated from crystallization pond on 16S rRNA gene sequence. 49
Figure 13. Survival curves of E. coli ATCC 51739 in reservoirs at different storage temperatures. 52
Figure 14. Survival curves of E. coli ATCC 51739 in concentrated brine reservoir at different storage temperatures. 53
Figure 15. Survival curves of E. coli ATCC 51739 in solar salt at different storage temperatures. 54
Figure 16. Survival curves of E. coli O157:H7 ATCC 43895 in reservoir at different storage temperatures. 57
Figure 17. Survival curves of E. coli O157:H7 ATCC 43895 in concentrated brine reservoir at different storage temperatures. 58
Figure 18. Survival curves of E. coli O157:H7 ATCC 43895 in solar salt salt at different storage temperatures. 59
Figure 19. Survival curves of S. aureus KCTC 1621 in reservoir at different storage temperatures. 62
Figure 20. Survival curves of S. aureus KCTC 1621 in concentrated brine reservoir at different storage temperatures. 63
Figure 21. Survival curves of S. aureus KCTC 1621 in solar salt at different storage temperatures. 64
Figure 22. Survival curves of S. Typhimurium KCTC 2515 in reservoirs at different storage temperatures. 67
Figure 23. Survival curves of S. Typhimurium KCTC 2515 in concentrated brine reservoir at different storage temperatures. 68
Figure 24. Survival curves of S. Typhimurium KCTC 2515 in solar salt at different storage temperatures. 69
Figure 25. Survival curves of L. monocytogenes 4244 in reservoirs at different storage temperatures. 72
Figure 26. Survival curves of L. monocytogenes 4244 in concentrated brine reservoir at different storage temperatures. 73
Figure 27. Survival curves of L. monocytogenes 4244 in solar salt at different storage temperatures. 74
Figure 28. Survival curves of S. sonnei KCTC 12004 in reservoirs at different storage temperatures. 77
Figure 29. Survival curves of S. sonnei KCTC 12004 in concentrated brine reservoir at different storage temperatures. 78
Figure 30. Survival curves of S. sonnei KCTC 12004 in solar salt at different storage temperatures. 79
Figure 31. Manufactured microplastics. 85
Figure 32. Filters used for the production of solar salt. 87
Figure 33. Simplicity test-bed of solar salt production. 89
Figure 34. Raman sepctra of an unknown particle in concentrated brine water(black) and its closest database match(orange). 92
Figure 35. Decomposing of particles after laser treatment by Raman. 93
Figure 36. Polymer distribution of the microparticles found in concentrated brine water(A~D saltern). 94
Figure 37. Polymer distribution of the microparticles found in concentrated brine water(E~H saltern). 95
Figure 38. Decreasing rate after filtration of the microplastic added in ephemeral saline reservoir by particle size analyzer. 98
Figure 39. Images of stained microplastic particles after filtration of concentrated brine water photographed under UV-light(365nm). 100
Figure 40. Photos under a fluorescence microscope with white light(left) and excitation emission wavelength of 470~525 nm(right) for the nile red... 101
Figure 41. Images of stained microplastic particles found in solar salt prepared by filtration photographed under UV-light (365 nm). 103
Figure 42. Photos under a fluorescence microscope with white light(left) and excitation emission wavelength of 470~525 nm(right) for the nile red... 104
Figure 43. Raman sepctra of an unknown particle in solar salt prepared by filtration(black) and its closest database match(orange). 106
Figure 44. Decomposing of particles after laser treatment by Raman. 107
Figure 45. Polymer distribution of the microparticles found in solar salt prepared by filtration. 108
Figure 46. Simplicity test-bed of solar salt production. 114
Figure 47. Brine water purification device(before). 118
Figure 48. Brine water purification device(after). 119
Figure 49. Fiber filter of brine water purification device. 120
Figure 50. Star pleated filter. 121
Figure 51. Particle size of solar salt produced using filter equipment. 132