Title Page
ABSTRACT
Contents
LIST OF ABBREVIATIONS 10
Ⅰ. INTRODUCTION 11
Ⅱ. MATERIALS AND METHODS 14
1. Yeast strains & growth media 14
2. Amplification of DNA fragment 14
3. Transformation of yeast cells 14
4. Analysis of HAC1 mRNA splicing 15
5. Real-time quantitative reverse transcription-PCR 15
6. Immunoblot analysis 16
7. Fluorescence microscopy 17
8. ChIP assay 17
9. rDNA silencing assay 18
10. rDNA recombination assay 19
Ⅲ. RESULTS 25
1. Loss of Arv1 induces UPR 25
2. Loss of Arv1 activates the MAP kinase Hog1 in UPR-dependent manner 27
3. Loss of Arv1 promotes the Msn2/4-Pnc1-Sir2 axis by activating Hog1 30
4. Ire1 and Hog1 are required for promoting rDNA stability in arv1△ 34
5. rDNA stability is enhanced during general ER stress 38
Ⅳ. DISCUSSION 42
Ⅴ. REFERENCES 45
국문 초록 49
Table 1. Strains used in this study 20
Table 2. Primers used in this study 23
Figure 1. The induction of UPR in arv1△ 26
Figure 2. Ire1-dependent Hog1 activation in arv1△ 28
Figure 3. Hog1-dependent nuclear accumulation of Msn2 and expression of Pnc1, leading to the enhancement of Sir2 association with rDNA region in arv1△ 32
Figure 4. Ire1, Hog1-dependent rDNA stability enhancement in arv1△ 36
Figure 5. Enhanced rDNA stability by PMT1 deletion and tunicamycin 39