Title Page
Abstract
Contents
Chapter 1. Introduction 9
1.1. Acetoin 10
1.2. Previous studies with producing acetoin 11
1.3. Production of (S)-acetoin in Saccharomyces cerevisiae 13
1.4. Research goals 16
Chapter 2. Materials and Methods 17
2.1. Strains and culture conditions 18
2.2. Plasmid construction 20
2.3. Analytical methods 23
Chapter 3. Results and Discussion 24
3.1. Selection of starting strain 25
3.2. Endogenous pyruvate decarboxylase PDC deletion 29
3.2.1. Single deletion 29
3.2.2. Double deletion 31
3.3. Diacetyl reductase ORA1 deletion 34
Chapter 4. Conclusions 36
References 39
초록 43
Table 1. Strains used in this study 19
Table 2. Primers used in this study 21
Table 3. Vectors used in this study 22
Figure 1. Acetoin production pathway in Saccharomyces cerevisiae 15
Figure 2. Acetoin production model in JHY901 and JHY901-9 26
Figure 3. Acetoin production model in JHYA100 (bdh1△) 28
Figure 4. Acetoin production in JHYA101 and JHYA103 30
Figure 5. Acetoin production in JHYA112 and JHYA113 32
Figure 6. Acetoin production in JHYA111 33
Figure 7. Acetoin production in JHYA200 35