Title Page

ABSTRACT

Contents

Chapter 1. Introduction 10

1.1. Research Background 10

Chapter 2. Experimental section and methods 12

2.1. Preparation of laser-etched molds 12

2.2. Synthesis of Gelatin-norbornene (GelNB) 12

2.3. Fabrication of Poly(ethylene glycol) diacrylate (PEGDA) hydrogels 16

2.4. Fabrication of GelNB hydrogels 18

2.5. Fabricaton of Poly acrylic acid (PAA) hydrogels 20

2.6. Preparation of Cryogels 22

2.7. Cryogels using the mold 22

2.8. Cryogels for bone regeneration 24

Chapter 3. Results and Discussion 26

3.1. Results 26

3.1.1. Hydrophobic properties of laser etched patterns 26

3.1.2. Hydrogel particles using the mold 29

3.1.3. Hydrogel particles made with various materials 31

3.1.4. Complex shapes with hydrogel particles 34

3.2. Discussion 36

Reference 38

국문초록 40

Figure 2.1. Schematic of GelNB synthesis. 14

Figure 2.2. Characterization of GelNB hydrogels. 15

Figure 2.3. Schematic representation of crosslinked PEGDA network. 17

Figure 2.4. Schematic representation of crosslinked GelNB network. 19

Figure 2.5. Schematic representation of crosslinked PAA network. 21

Figure 2.6. Cryogel fabrication process scheme. 23

Figure 2.7. Fabrication of GelNB hydrogel particles for bone regeneration scaffolds. 25

Figure 3.1. SEM images of the laser-etched mold. 27

Figure 3.2. Contact angles of the laser-etched mold. 28

Figure 3.3. Changes in mold conditions after repeated hydrogel casting. 30

Figure 3.4. SEM images of cryogels made with various materials. 32

Figure 3.5. Changes in the size of various materials by the casting process. 33

Figure 3.6. Application with complex shapes. 35

Figure 3.7. Sample implantation procedure for bone regeneration in vivo study. 37