표제지
국문요약
목차
서론 11
연구 대상 및 방법 13
1. 조사 대상 및 방법 13
2. 임상적 특징 및 정의 13
3. 미생물학적 검사 14
4. 면역학적 검사 14
4.1. 혈장에서 시토카인의 측정 14
4.2. 말초혈액단핵구에서 IFN-γ 측정 14
5. 재발성 S. aureus 균주와 비재발성 S. aureus 균주의 mprF와 dltA 발현 분석 15
6. 통계 방법 15
연구 결과 17
1. 연구 대상 17
2. 재발성 S. aureus 균혈증 환자의 임상적 특성 19
3. 재발성 S. aureus 균혈증 균주의 특성 19
4. 혈장내 IFN-γ 농도 비교 24
4.1. 재발성 균혈증 환자와 비재발성 균혈증 환자간의 IFN-γ 농도 비교 24
4.2. 연속적 혈액 검체에서 IFN-γ 농도 비교 24
4.3. 임상 변수에 따른 IFN-γ 비교 30
4.4. 말초혈액단핵구에서 IFN-γ 세포내 염색 검사 결과 30
5. 혈장내 IL-6, IL-10, IL-17A 및 TNF-α 농도 비교 34
6. 재발성 S. aureus 균주와 비재발성 S. aureus 균주 간 mprF와 dltA 발현 비교 34
고찰 38
결론 44
참고문헌 45
Abstract 51
Table 1. Sequence of primers used in the quantitative reverse transcription polymerase chain reaction 15
Table 2. Comparison of clinical characteristics between recurrent S. aureus bacteremia patients and non-recurrent S. aureus bacteremia patients 20
Table 3. Comparison of microbiological characteristics between recurrent S. aureus bacteremia and non-recurrent S. aureus bacteremia 22
Table 4. Comparison of microbiological characteristics of methicillin-resistant S. aureus strain between recurrent S. aureus bacteremia and non-recurrent S. aureus bacteremia 23
Table 5. Summary of clinical details of recurrent S. aureus bacteremia patients who had blood samples before and after bacteremia clearance 28
Table 6. Intracellular cytokine staining of peripheral blood mononuclear cell of recurrent S. aureus bacteremia 32
Figure 1. Summary of patients included in the present study. 18
Figure 2. Comparison of plasma concentration of IFN-γ between recurrent and non-recurrent S. aureus bacteremia. 25
Figure 3. Summary of the duration of bacteremia and timing of blood samples in initial S. aureus bacteremia episode of recurrent S. aureus bacteremia patients. 26
Figure 4. Comparison of plasma concentration of IFN-γ between recurrent and non-recurrent S. aureus bacteremia according to bacteremia clearance. 27
Figure 5. Changes of plasma concentration of IFN-γ in recurrent S. aureus bacteremia patients who had blood samples before and after bacteremia clearance. 29
Figure 6. Comparison of plasma concentration of IFN-γ between recurrent and non-recurrent S.aureus bacteremia according to bacteremia clearance in the patients with the serial blood samples. 31
Figure 7. Comparison of plasma concentration of IFN-γ according to the clinical factors. 33
Figure 8. Comparison of plasma concentration of IL-6, IL-10, IL-17, and TNF-α between recurrent and non-recurrent S. aureus bacteremia patients before bacteremia clearance. 35
Figure 9. Comparison of plasma concentration of IL-6, IL-10, IL-17, and TNF-α between recurrent and non-recurrent S. aureus bacteremia patients after bacteremia clearance. 36
Figure 10. Comparison of mprF and dltA expression between recurrent and non-recurrent S. aureus bacteremia patients. 37