Title Page
영문 요약
Contents
Introduction 11
Methods 13
Results 22
Discussion 46
Conclusion 51
References 52
국문 요약 58
Table 1. The detail of primers used in the Real-time PCR analysis 20
Table 2. Yield, chemical composition, monosaccharide content and molecular weight of polysaccharides extracted from Cnidium officinale 24
Table 3. Glycosidic linkage analysis of F₂ 45
Fig. 1. (i) Elution profile of crude polysaccharide from a DEAE-Sepharose fast flow column chromatography. (ii) Molecular characterization of polysaccharides obtained... 26
Fig. 2. (A) Effect of polysaccharides on the proliferation of RAW264.7 cells. RAW264.7 cells (1×10⁶ cells/well) were incubated for 24 h with different concentrations of... 28
Fig. 3. The mRNA expression levels of iNOS, TNF-α, IL-1β, IL-6, and IL-10 determined by Real-Time PCR. LPS was used as a control. 1 μg/mL of LPS as the... 30
Fig. 4. Effect of polysaccharides obtained from C. officinale on the p-NF-κB, p-JNK, p-ERK ½, and p-p38 proteins expression in RAW264.7 cells. 32
Fig. 5. The antibody neutralization assay carried out by measuring the NO production. 34
Fig. 6. Effect of polysaccharides on the proliferation of NK-92 and HCT-116 cells. Cells (1×10⁶ cells/well) were incubated for 24 h with different concentrations of... 36
Fig. 7. Cytotoxicity of NK cells against HT-29 cells with the WST-1 assay. Data are presented as the mean ± standard deviation (n=3). 38
Fig. 8. The mRNA expression levels of TNF-α, IFN-γ, NKGp44, Granzyme-B determined by Real-Time PCR. Values were presented as mean ± standard deviations (n=3). 40
Fig. 9. Effect of polysaccharides obtained from C. officinale on the p-NF-κB, p-JNK, p-ERK ½, and p-p38 proteins expression in Nk cells. 42
Fig. 10. NK cell cytotoxicity against HCT-116 cells in an antibody neutralization assay. Values are presented as mean ± standard deviation (n=3). 43