Title Page
영문요약
Contents
1. INTRODUCTION 7
2. MATERIALS AND METHODS 8
2.1. Case selection 8
2.2. Histopathologic evaluation of lymph nodes 8
2.3. Tissue microarray construction 9
2.4. Immunohistochemical analysis 9
2.5. Statistical analysis 10
3. RESULTS 11
3.1. Study cohort characteristics 11
3.2. Clinicopathologic characteristics association with ENE status 11
3.3. Association with ENE and number of metastatic nodes and size of metastatic foci 15
3.4. Survival analysis according to ENE status 15
3.5. Prognostic factors for ampullary adenocarcinoma 17
3.6. Expression of EMT markers in the areas with and without ENE 21
4. Discussion 25
REFERENCES 29
국문요약 32
Table 1. Clinicopathologic characteristics of 279 patients with ampullary adenocarcinoma 13
Table 2. Clinicopathologic characteristics of 94 patients with ampullary adenocarcinoma and lymph node metastasis based on ENE status 13
Table 3. Association with the ENE status and local recurrence, and distant metastasis 17
Table 4. Univariable Cox regression analysis in patients with 279 Ampullary adenocarcinoma patients 18
Table 5. Multivariable Cox regression analysis in patients with 279 Ampullary adenocarcinoma patients 19
Table 6. Univariable Cox regression analysis in patients with 95 Ampullary adenocarcinoma patients with nodal metastasis 20
Table 7. Multivariable Cox regression analysis in patients with 95 Ampullary adenocarcinoma patients with nodal metastasis 21
Figure 1. Representative histologic images of lymph node metastasis based on ENE status. Lymph node metastasis (A) with ENE and (B) without ENE (magnification, all, x 50). 11
Figure 2. Kaplan-Meier survival analyses. (A) Ampullary adenocarcinoma patients with nodal metastasis and ENE had significantly worse 5-year OS than those with nodal metastasis without ENE... 16
Figure 3. Representative images of (A, F) H&E staining, (B, G) E-cadherin, (C, H) Twist, (D, I) Snail, and (E, J) Cytokeratin immunohistochemical staining in nodal metastatic cancer cells in the area with... 23
Figure 4. Comparison of EMT markers expression in nodal metastatic cancer cells in areas with and without ENE. (A) The ROC curve for E-cadherin, Twist, and Snail. The violin plots overlaid with dot plots... 24