Title Page
Abstract
Contents
Introduction 8
Materials and Methods 10
1. Cell culture and Reagent 10
2. Cell viability Assay 10
3. Western blotting 10
4. Oligomerization 11
5. Proliferation assay 11
6. Flow cytometric analysis 11
7. RNA extraction and RT-PCR analysis 11
8. TUNEL assay 12
9. Confocal Microscopy 13
10. Migration Assay 13
Results 14
1. WYE - 125132 inhibits cell viability and cell proliferation in glioblastoma cells 14
2. WYE- 125132 induce not apoptosis but cell cycle arrest in glioblastoma cells 17
3. WYE - 125132 inhibits mTOR activation and decrease phosphorylation of AKT in glioblastoma cells 21
4. WYE - 125132 inhibits cell proliferation in glioblastoma cells 24
5. TFEB is expressed in glioblastoma cells 28
6. WYE-125132 suppresses mTOR pathway and regulates TFEB in glioblastoma cells 30
7. WYE - 125132 inhibits TFEB oligomerization in glioblastoma cells 32
8. WYE - 125132 inhibits TFEB oligomerization in glioblastoma cells 34
9. WYE-125132 represses mTOR and proliferation in shTFEB U87MG 36
10. The addition of Temozolomide to WYE-125132 enhances the apoptosis in glioblastoma cells 39
Discussion 42
Conclusion 44
Reference 45
Abstract (in Korean) 48
Figure 1. WYE - 125132 inhibits cell viability and cell proliferation in glioblastoma cells 15
Figure 2. WYE- 125132 induces not apoptosis but cell cycle arrest in glioblastoma cells 20
Figure 3. WYE - 125132 inhibits mTOR activation and decrease phosphorylation of AKT in glioblastoma cells 23
Figure 4. WYE - 125132 inhibits cell proliferation in glioblastoma cells 27
Figure 5. TFEB is expressed in glioblastoma cells 29
Figure 6. WYE-125132 suppresses mTOR pathway and regulates TFEB in glioblastoma cells 31
Figure 7. WYE - 125132 inhibits TFEB oligomerization in glioblastoma cells 33
Figure 8. WYE - 125132 inhibits TFEB oligomerization in glioblastoma cells 35
Figure 9. WYE-125132 represses mTOR and proliferation in shTFEB U87MG 38
Figure 10. The addition of Temozolomide to WYE-125132 enhances the apoptosis in glioblastoma cells 41