표제지
목차
국문 초록 12
I. 이론적 배경 15
II. 재료 및 방법 19
1. 새싹삼 및 생산농가환경 미생물 오염도 조사 19
1.1. 실험 재료 19
1.2. 위생지표세균 정량분석 23
1.3. 병원성 미생물 분석 25
2. 분리된 Bacillus cereus 독소 유전자 분석 31
2.1. 실험재료 31
2.2. 실험방법 31
3. 포장용 이끼에 오염된 Bacillus cereus 저감화 34
3.1. 실험 재료 34
3.2. 실험방법 37
4. 통계 분석 38
III. 결과 및 고찰 40
1. 오염도 조사 40
1.1. 새싹삼과 포장용이끼의 유해미생물 오염도 조사 40
1.2. 새싹삼 생산 환경 유해미생물 오염원 조사 49
2. Bacillus cereus 독소 유전자 분석 63
3. 포장용 이끼에 오염된 Bacillus cereus 제어 효과 66
3.1. 미산성전해수 및 열 건조 단독 또는 병용 처리 효과 66
3.2. 저장안전성평가 68
IV. 결론 72
Abstract 74
참고문헌 78
Table 1. Characteristics of ginseng sprout used in this study 20
Table 2. Target gene and amplicon size(bp) for PCR used in this study 29
Table 3. Culture media and identification methods for foodborne pathogen isolated from ginseng sprout and moss used in this study 30
Table 4. Bacillus cereus Toxin Real-time PCR run protocol. 32
Table 5. Bacillus cereus toxin related properties. 33
Table 6. Populations (log10 CFU/g) of total aerobic bacteria, Escherichia coli, coliform, yeast, and mold in ginseng sprout produced by different farms[이미지참조] 42
Table 7. Populations (log10 CFU/g) of total aerobic bacteria, Escherichia coli, coliform, yeast, and mold in moss produced by different farm[이미지참조] 43
Table 8. Identification of pathogenic bacteria isolated from green moss produced by 20 different farm 46
Table 9. Populations (log10 CFU/g) of Bacillus cereus on ginseng sprouts and moss[이미지참조] 48
Table 10. Contamination levels (log CFU/g) of total aerobic bacteria in the samples collected from environment during cultivation of ginseng sprout in A, B, and C farms 51
Table 11. Contamination levels (log CFU/g) of coliform in the samples collected from environment during cultivation of ginseng sprout in A, B, and C farms 53
Table 12. Contamination levels (log CFU/g) of yeast in the samples collected from environment during cultivation of ginseng sprout in A, B, and C farms 55
Table 13. Contamination levels (log CFU/g) of mold in the samples collected from environment during cultivation of ginseng sprout in A, B, and C farms 57
Table 14. Populations (log10 CFU/g) of Bacillus cereus group on ginseng sprout farm cultivation, production environments and working tool. 61
Table 15. Analysis of isolated Bacillus cereus toxins based on monitoring results. 64
Table 16. Reduction of green moss for packaging using HAEW inoculated with the Bacillus cereus (rif) strain. 69
Table 17. Reduction of green moss for packaging using HAEW and heat dry inoculated with the Bacillus cereus (rif) strain 70
Figure 1. Ginseng sprout farms cultivation step of sample list 22
Figure 2. Slightly Acidic Electrolyzed Water(HAEW) making device 36
Figure 3. Reduction of green moss for packaging using slightly acidic electrolyzed water (SAEW) and heat dry method 39
Figure 4. Populations (log10 CFU/g) of Bacillus cereus group on ginseng sprout (A) and green moss (B). N, Q, and T farms for green moss not tested. Values represent the mean±SD (n=3). Mean with different letters above a bar significantly different (p〈0.05) by Tukey multiple range test. 47
Figure 5. Storage safety experiment after combined treatment with packaging green moss. (A); control group - sterilized distilled water, (B); treatment group - slightly acidic electrolyzed water. Values represent the mean±SD (n=3). Mean with different letters above a bar significantly different (p〈0.05) by Tukey multiple range test. 71